Dopamine regulation of [3H]acetylcholine release from guinea-pig stomach.
Dopamine regulation of [3H]acetylcholine release from guinea-pig stomach.
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发表时间：
1985-09
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通讯作者：
M. Kusunoki;K. Taniyama;C. Tanaka
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作者：
M. Kusunoki;K. Taniyama;C. Tanaka
通过分析多巴胺受体激动剂和拮抗剂对豚鼠胃乙酰胆碱（ACh）释放的影响，探讨多巴胺受体参与豚鼠胃胆碱能传递。电刺激（1-20 Hz）预载[~ 3 H]胆碱的豚鼠胃条引起[~ 3 H]ACh释放，该释放是钙依赖性的和河豚毒素敏感的。多巴胺以浓度依赖性方式（10（-8）-10（-4）M）抑制这种跨壁刺激诱导的[3 H]ACh释放。10（-5）M六烃季铵不改变多巴胺的这种作用，从而表明主要的多巴胺受体位于节后胆碱能神经元上。多巴胺对[~ 3 H]ACh释放的浓度-反应曲线可被氟哌啶醇、舒必利和多潘立酮抑制，但不被哌唑嗪、育亨宾、普萘洛尔和酮色林抑制。D2多巴胺受体激动剂LY 171555在一定程度上减少了透壁刺激引起的[3 H]ACh释放，而D1多巴胺受体激动剂SKF 38-393则没有这种作用。鉴于上述结果，ACh从节后胆碱能神经元的释放可能需要通过多巴胺受体拮抗剂D2，而不是肾上腺素能或5-羟色胺受体拮抗剂拮抗。
The involvement of dopamine receptors in cholinergic transmission of guinea-pig stomach was investigated by analyzing the effects of dopamine receptor agonists and antagonists on acetylcholine (ACh) release from this organ. Electrical stimulation (1-20 Hz) of strips of guinea-pig stomach preloaded with [3H] choline induced a [3H]ACh release that was calcium dependent and tetrodotoxin sensitive. Dopamine inhibited this transmural stimulation-induced [3H]ACh release in a concentration-dependent manner (10(-8)-10(-4) M). This effect of dopamine was not altered by 10(-5) M hexamethonium, thereby suggesting that the major dopamine receptors are located on the postganglionic cholinergic neurons. Concentration-response curves for dopamine on [3H]ACh release were inhibited by haloperidol, sulpiride and domperidone but not by prazosin, yohimbine, propranolol and ketanserin. LY 171555, an agonist for the D2 dopamine receptor, but not SKF 38-393, an agonist for the D1 dopamine receptor, to some extent decreased the release of [3H]ACh induced by transmural stimulation. In view of the results, the release of ACh from postganglionic cholinergic neurons is probably required through dopamine receptors antagonized by D2 antagonists but not by adrenergic or serotonin receptor antagonists.