Modular aptameric sensors
Modular aptameric sensors
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DOI：
10.1021/ja032013t
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发表时间：
2004-08-04
影响因子：
15
通讯作者：
Kolpashchikov, DM
中科院分区：
文献类型：
--
作者：
Stojanovic, MN;Kolpashchikov, DM
我们报告的第一个例子的模块化适体传感器，通过变构调节与荧光团的非共价相互作用，将识别事件转化为荧光变化。这些传感器包括：（a）报告结构域，其通过与荧光团结合而发出分析物结合事件的信号;（B）识别结构域，其结合分析物;和（c）通信模块，其充当识别结构域和信号传导结构域之间的管道。我们测试了识别区域的ATP，FMN，和茶碱与孔雀石绿色结合适体作为信号传导域的组合。在每种情况下，我们都能够获得一个功能传感器，能够响应分析物浓度的增加，荧光的增加。仅由天然RNA组成的类似构建体可以在细胞中表达，并用作细胞内成像的传感器。
We report the first examples of modular aptameric sensors, which transduce recognition events into fluorescence changes through allosteric regulation of noncovalent interactions with a fluorophore. These sensors consist of: (a) a reporting domain, which signals the binding event of an analyte through binding to a fluorophore; (b) a recognition domain, which binds the analyte; and (c) a communication module, which serves as a conduit between recognition and signaling domains. We tested recognition regions specific for ATP, FMN, and theophylline in combinations with malachite green binding aptamer as a signaling domain. In each case, we were able to obtain a functional sensor capable of responding to an increase in analyte concentration with an increase in fluorescence. Similar constructs that consist only of natural RNA could be expressed in cells and used as sensors for intracellular imaging.