Hepatic Regeneration after Sublethal Partial Liver Irradiation in Cirrhotic Rats
Hepatic Regeneration after Sublethal Partial Liver Irradiation in Cirrhotic Rats
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肝硬化大鼠亚致死部分肝脏照射后的肝脏再生
DOI：
10.1269/jrr.11002
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发表时间：
2011-09-01
影响因子：
2
通讯作者：
Jiang, Guo-Liang
中科院分区：
文献类型：
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作者：
Gu, Ke;Lai, Song-Tao;Jiang, Guo-Liang
我们先前的动物研究已经证明部分肝脏照射（IR）可以刺激受保护肝脏的再生，这支持了肝癌放射治疗计划中所采用的措施。本研究旨在探讨部分肝缺血再灌注是否能诱发大鼠肝硬化再生。肝硬化建立后，停用TAA。实验Ⅰ仅照射右半肝，剂量分别为5戈伊、10戈伊和15戈伊。实验2右半肝照射剂量为15戈伊，左半肝照射剂量分别为2.5戈伊、5戈伊和7.5戈伊。分别于术后0、30、60、90、120、150 d检测肝指数（LI）、有丝分裂指数（MI）、肝增殖指数（LPI）、PCNA标记指数（PCNA-LI）、血清HGF、VEGF、TGF-α和IL-6，并检测血清和原位TGF-β 1水平。在两个实验组中，IR损伤是亚致死的，诱导不超过9%的动物死亡。TAA停药后，对照组肝再生减慢。在实验1中，除了LI，所有其他再生参数均显着高于对照组的右半肝和左半肝。在实验2中，所有再生参数也高于对照组的两个半肝。血清HGF、VEGF水平较对照组明显升高。未照射和低剂量照射的缺血肝均能由亚致死的部分肝IR触发再生，高剂量和对两半肝的IR触发更强的再生。
Our previous animal study had demonstrated that partial liver irradiation (IR) could stimulate regeneration in the protected liver, which supported the measurements adopted in radiotherapy planning for hepatocellular carcinoma. The purpose of this present study is to investigate whether cirrhotic liver repopulation could be triggered by partial liver IR. The cirrhosis was induced by thioacetamide (TAA) in rats. After cirrhosis establishment, TAA was withdrawn. In Experiment I, only right-half liver was irradiated with single doses of 5 Gy, 10 Gy and 15 Gy, respectively. In Experiment 2, right-half liver was irradiated to 15 Gy, and the left-half to 2.5 Gy, 5 Gy and 7.5 Gy, respectively. The regeneration endpoints, including liver index (LI); mitotic index (MI); liver proliferation index (LPI); PCNA-labeling index (PCNA-LI); serum HGF, VEGF, TGF-alpha and IL-6, were evaluated on 0 day, 30-day, 60-day, 90-day, 120-day and 150-day after IR. Serum and in situ TGF-beta 1 were also measured. In both experimental groups, the IR injuries were sublethal, inducing no more than 9% animal deaths. Upon TAA withdrawal, hepatic regeneration decelerated in the controls. In Experiment 1 except for LI, all other regeneration parameters were significantly higher than those in controls for both right-half and left-half livers. In Experiment 2 all regeneration parameters were also higher compared with those in controls for both half livers. Serum HGF and VEGF were increased compared with that of controls. Both unirradiated and low dose-irradiated cirrhotic liver were able to regenerate triggered by sublethal partial liver IR and higher doses and IR to both halves liver triggered a more enhanced regeneration.