The arrangement of F-actin and microtubules during germination of Mucor rouxii sporangiospores.
The arrangement of F-actin and microtubules during germination of Mucor rouxii sporangiospores.
复制标题
鲁氏毛霉孢子萌发过程中 F-肌动蛋白和微管的排列。
DOI：
10.1007/bf00288945
复制
发表时间：
1994
影响因子：
2.8
通讯作者：
Bartnicki-Garcia,S
中科院分区：
文献类型：
--
作者：
Hasek,J;Bartnicki-Garcia,S
用罗丹明标记的鬼笔环肽标记和免疫荧光显微镜观察了F-actin微丝和微管在鲁氏毛霉孢子囊孢子萌发过程中的分布。从等径到顶端生长的过渡是伴随着从均匀分布的F-肌动蛋白补丁的极化积累的F-肌动蛋白材料在芽管尖端的开关。M.无细胞提取物的免疫印迹rouxii与抗猪α-微管蛋白单克隆抗体（TU-01）杂交，发现α-微管蛋白有两条不连续的带，提示该真菌中存在两个α-微管蛋白基因。用相同的抗体染色的萌发细胞的免疫荧光显微镜显示了一个精心制作的细胞质微管网络，坚持在整个萌发过程中，并延伸到芽管的顶点。虽然他们的确切作用仍然未定，在萌发过程中观察到的细胞骨架元素的安排是一致的，他们假定参与细胞壁形态发生：长轴微管作为长距离的输送机壁建设囊泡的顶端区域，而集中的F-肌动蛋白补丁标记参与微丝在区强烈的囊泡胞吐在菌丝顶端。
The distribution of F-actin microfilaments and microtubules was analyzed in germinating sporangiospores ofMucor rouxiiby labeling with rhodamine-tagged phalloidin and by immunofluorescence microscopy. The transition from isodiametrical to apical growth was accompanied by a switch from uniform distribution of F-actin patches to a polarized accumulation of F-actin material at the germ tube tips. Immunoblotting of cell-free extracts ofM. rouxiiwith a monoclonal anti-porcine α-tubulin antibody (TU-01) disclosed two discrete bands of α-tubulin suggesting the existence of two α-tubulin genes in this fungus. Immunofluorescence microscopy of germinating cells stained with the same antibody revealed an elaborate network of cytoplasmic microtubules that persisted during the entire germination process and extended into the apex of the germ tube. Although their precise roles remain undetermined, the observed arrangement of cytoskeletal elements during germination is consistent with their presumed involvement in cell wall morphogenesis: the long axial microtubules serving as long-distance conveyors of wall-building vesicles to the apical region while the concentrated F-actin patches mark the participation of microfilaments in the zone of intense vesicle exocytosis at the hyphal apex.