Cell surface interaction induces polarization of mouse 8-cell blastomeres at compaction
Cell surface interaction induces polarization of mouse 8-cell blastomeres at compaction
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DOI：
10.1016/0092-8674(80)90457-2
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发表时间：
1980-10
期刊：
影响因子：
64.5
通讯作者：
C. Ziomek;M. Johnson
中科院分区：
文献类型：
--
作者：
C. Ziomek;M. Johnson
本文研究了荧光标记配体伴刀豆球蛋白A（FITC Con A）在小鼠早期胚胎卵裂球表面极化结合的发育。单个S-细胞卵裂球，天然的g-细胞偶联衍生自体外分裂的个人4-细胞卵裂球，并从解离的S-细胞制成的重新聚集的偶联培养不同的时间段和各种基质。表面极性的发展被认为是高度依赖于细胞接触。在培养4-5小时后，偶联中超过50%的细胞极化，偶联中较小的细胞通常比较大的细胞极化更早。FITC-Con A结合的极点的方向与偶联中细胞之间的接触点相反，而不管它们先前在胚胎或卵裂平面内的方向如何。
The development of the polarized surface binding of the fluoresceinated ligand concanavalin A (FITC Con A) was studied in blastomeres of the early mouse embryo. Single S-cell blastomeres, natural g-cell couplets derived from the in vitro division of individual 4-cell blastomeres, and reaggregated couplets made from dissociated S-cells were cultured for varying periods of time and on a variety of substrata. The development of surface polarity was found to be highly dependent upon cell contact. Over 50% of the cells in couplets were polarized after 4-5 hr in culture, with the smaller cell in the couplet usually more advanced in its polarization than the larger cell. The orientation of the poles of FITC-Con A binding was opposite the point of contact between cells in the couplets regardless of their previous orientation within the embryo or the plane of cleavage.