Electrocatalytic oxidation of NADH at gold nanoparticles loaded poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) film modified electrode and integration of alcohol dehydrogenase for alcohol sensing
Electrocatalytic oxidation of NADH at gold nanoparticles loaded poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) film modified electrode and integration of alcohol dehydrogenase for alcohol sensing
复制标题
DOI：
10.1016/j.talanta.2008.01.038
复制
发表时间：
2008-06-15
期刊：
影响因子：
6.1
通讯作者：
Lee, K. P.
中科院分区：
文献类型：
--
作者：
Manesh, K. M.;Santhosh, P.;Lee, K. P.
将金纳米粒子分散在聚(3，4-乙二氧基噻吩基)-聚苯乙烯磺酸(PEDOT-PSS)基质上，制备了一种新型的修饰电极。研究了PEDOT-PSS-Au-纳米电极对β-烟酰胺腺嘌呤二核苷酸(NADH)氧化的电催化活性。与PEDOT-PSS电极相比，NADH在PEDOT-PSS-Au-Nano电极上的氧化过电位显著降低(>；0.7V)。分散在PEDOT-PSS中的Au纳米颗粒可防止NADH氧化产物对电极表面的污染，并在较低的正电位(+0.04V vs.SCE)下增强NADH的氧化。该电极对NADH的电催化氧化具有很高的灵敏度。此外，在检测NADH时，抗坏血酸和尿酸的存在不会干扰。在PEDOT-PSS-Au-Nano电极上实现了电极的稳定性(20天后仍保持原始活性的95%)、测量的重现性(相对标准偏差：2.8%；n=5)、选择性和宽的线性动态范围(1-80µM；R-2=0.996)等重要的实用优点。由于PEDOT-PSS-Au-Nano电极能够促进NADH与电极之间的电子传递，因此我们构建了一种生物相容的乙醇脱氢酶生物传感器。该传感器对乙醇具有较高的灵敏度，检测速度快，重现性好，稳定性好。(C)2008爱思唯尔B.V.保留所有权利。
A new modified electrode is fabricated by dispersing gold nanoparticles onto the matrix of poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid), PEDOT-PSS. The electrocatalytic activity of the PEDOT-PSS-Au-nano electrode towards the oxidation of beta-nicotinamide adenine dinucleotide (NADH) is investigated. A substantial decrease in the overpotential (>0.7 V) has been observed for the oxidation of NADH at the PEDOT-PSS-Au-nano electrode in comparison to the potential at PEDOT-PSS electrode. The Au nanoparticles dispersed in the PEDOT-PSS matrix prevents the fouling of electrode surface by the oxidation products of NADH and augments the oxidation of NADH at a less positive potential (+0.04 V vs. SCE). The electrode shows high sensitivity to the electrocatalytic oxidation of NADH. Further, the presence of ascorbic acid and uric acid does not interfere during the detection of NADH. Important practical advantages such as stability of the electrode (retains similar to 95% of its original activity after 20 days), reproducibility of the measurements (R.S.D.: 2.8%; n = 5), selectivity and wide linear dynamic range (1-80 mu M; R-2 = 0.996) are achieved at PEDOT-PSS-Au-nano electrode. The ability of PEDOT-PSS-Au-nano electrode to promote the electron transfer between NADH and the electrode makes us to fabricate a biocompatible dehydrogenase-based biosensor for the measurement of ethanol. The biosensor showed high sensitivity to ethanol with rapid detection, good reproducibility and excellent stability. (C) 2008 Elsevier B.V. All rights reserved.