The expression of NOB1 in spiral ganglion cells of guinea pig
The expression of NOB1 in spiral ganglion cells of guinea pig
复制标题
NOB1在豚鼠螺旋神经节细胞中的表达。
DOI：
10.1016/j.ijporl.2008.10.028
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发表时间：
2009-02-01
影响因子：
1.5
通讯作者：
Qiu, Jianhua
中科院分区：
文献类型：
--
作者：
Han, Yu;Hong, Liu;Qiu, Jianhua
目的：NOB 1是一种转录相关蛋白，由一个锌带结构域组成。本研究旨在探讨NOB 1在正常豚鼠和耳聋豚鼠耳蜗螺旋神经节细胞中的表达。实验组给予庆大霉素单次肌肉注射，对照组给予生理盐水。分别于注射前和注射后10天进行听性脑干反应（ABR）测试。处死两组豚鼠，取出颞骨。结果：NOB 1在耳蜗基底回至顶回的螺旋神经节细胞均有表达。NOB 1在聋豚鼠耳蜗螺旋神经节细胞中的表达较正常豚鼠明显增强。结论：NOB 1在聋豚鼠耳蜗螺旋神经节细胞中存在，可能参与听觉信号的传导。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Objective: NOB1 was a transcription-associated protein, consisting of one zinc ribbon domain. This study aimed to investigate the NOB1 expression in spiral ganglion cells of normal guinea pigs and deaf ones.Methods: Twelve guinea pigs were randomized equally into experimental group and control group. In experimental group, guinea pigs received a single intramuscular injection of gentamicin, while the control group was treated with physiological saline. Auditory brainstem responses (ABR) test was performed before and 10 days after injection, respectively. Guinea pigs of both groups were sacrificed and temporal bones were removed. The expression of NOB1 in the spiral ganglion was evaluated by immunohistochemistry.Results: NOB1 staining was found expressed in spiral ganglion cells from the basal turn to the apical turn of the cochlea. The expression of NOB1 was found significantly stronger in spiral ganglion cells of deaf guinea pigs as compared with that in normal ones.Conclusions: The NOB1 was presented in spiral ganglions cells of the guinea pig and might play a role in hearing transduction. (C) 2008 Elsevier Ireland Ltd. All rights reserved.