An Additive Effect of Promoting Thermogenic Gene Expression in Mice Adipose-Derived Stromal Vascular Cells by Combination of Rosiglitazone and CL316,243.
An Additive Effect of Promoting Thermogenic Gene Expression in Mice Adipose-Derived Stromal Vascular Cells by Combination of Rosiglitazone and CL316,243.
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DOI：
10.3390/ijms18051002
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发表时间：
2017-05-08
影响因子：
5.6
通讯作者：
Yang GS
中科院分区：
文献类型：
--
作者：
Li YL;Li X;Jiang TT;Fan JM;Zheng XL;Shi XE;Yu TY;Chu GY;Yang GS
有充分证据表明，CL 316，243（β3激动剂）或罗格列酮（PPARγ激动剂）可诱导白色脂肪细胞群变为棕色样脂肪细胞，从而增加能量消耗并对抗肥胖。然而，是否存在联合效应仍然未知。本研究以小鼠腹股沟白色脂肪组织基质血管细胞（iWAT-SVCs）为材料，分别用CL 316、243、罗格列酮或二者联合诱导细胞布朗宁。结果显示，与单独使用CL 316，243或罗格列酮相比，CL 316，243和罗格列酮的组合显著上调核心产热基因Ucp 1以及与线粒体功能相关的基因（Cidea，Cox 5 b，Cox 7a 1，Cox 8b和Cycs）的表达。此外，与罗格列酮共同治疗可以逆转单独由CL 316，243刺激引起的脂联素下调。总之，罗格列酮和CL 316，243的组合可以产生促进小鼠iWAT-SVC中的产热基因表达和改善胰岛素敏感性的累加效应。
It is well-documented that CL316,243 (a β3 agonist) or rosiglitazone (a PPARγ agonist) can induce white adipocyte populations to brown-like adipocytes, thus increasing energy consumption and combating obesity. However, whether there is a combined effect remains unknown. In the present study, stromal vascular cells of inguinal white adipose tissue (iWAT-SVCs for short) from mice were cultured and induced into browning by CL316,243, rosiglitazone, or both. Results showed that a combination of CL316,243 and rosiglitazone significantly upregulated the expression of the core thermogenic gene Ucp1 as well as genes related with mitochondrial function (Cidea, Cox5b, Cox7a1, Cox8b, and Cycs), compared with the treatment of CL316,243 or rosiglitazone alone. Moreover, co-treatment with rosiglitazone could reverse the downregulation of Adiponectin resulting from CL316,243 stimuli alone. Taken together, a combination of rosiglitazone and CL316,243 can produce an additive effect of promoting thermogenic gene expression and an improvement of insulin sensitivity in mouse iWAT-SVCs.