Organically modified silica sol-mediated capillary electrophoresis
Organically modified silica sol-mediated capillary electrophoresis
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DOI：
10.1021/ac015718r
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发表时间：
2002-07-15
影响因子：
7.4
通讯作者：
Lev, O
中科院分区：
文献类型：
--
作者：
Neiman, B;Grushka, E;Lev, O
在本文中，我们描述了使用ormosil（有机改性二氧化硅）溶胶作为毛细管电泳中溶质之间选择性操作的运行缓冲液的添加剂。在运行缓冲液中含有溶胶添加剂的CE系统可以被认为是假毛细管电色谱。研究了三种基于不同类型硅烷的溶胶。甲基三甲氧基硅烷（MTMOS）基溶胶提高了各种芳香酸之间的选择性。氨基丙基三甲氧基硅烷（APS）溶胶与芳香酸结构异构体的相互作用不同于MTMOS。在运行缓冲液中加入APS溶胶的低pH下，也可以分离中性溶质。APS和N-[3-（三甲酰基）丙基]-乙二胺（EDAS）对同一化合物的分离影响不同，这表明即使是溶胶中官能团的微小变化也会对溶胶与溶质之间的相互作用产生显著的影响。
We describe in this paper the use of ormosil (organically modified silica) sols as additives to the run buffer for selectivity manipulations between solutes in capillary electrophoresis. CE systems that contain Sol additives in the run buffer can be thought of as pseudocapillary electrochromatography. Three sols based on different types of silanes were studied. Methyltrimethoxysilane (MTMOS)-based sol was found to improve selectivities between various aromatic acids. Aminopropyltrimethoxysilane (APS) sol interacts differently with structural isomers of aromatic acids than does MTMOS. At low pH with APS sol in the run buffer, neutral solutes can be separated, as well. The separation of the neutral solutes seems to be facilitated by the formation of hydrogen bonds between the solutes and the APS sol. APS and N-[3-(trimethoVsilyl)propyl]-ethylenediamine (EDAS) affect the separation of the same compounds differently, thus indicating that even small changes of the functional groups of the Sol have pronounced effect on the interactions between the sols and the solutes.