Expression of the kynurenine enzymes in macrophages and microglial cells: regulation by immune modulators
Expression of the kynurenine enzymes in macrophages and microglial cells: regulation by immune modulators
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DOI：
10.1007/bf01345271
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发表时间：
1998-01-01
期刊：
影响因子：
3.5
通讯作者：
Cesura, AM
中科院分区：
文献类型：
--
作者：
Alberati-Giani, D;Cesura, AM
在克隆的小鼠巨噬细胞（MT 2）和小胶质细胞（N11）中研究了吲哚胺2，3-双加氧酶（IDO）表达的调节。两种细胞系在干扰素-γ（IFN-γ）刺激后表达IDO和诱导型一氧化氮合酶活性。IDO表达的调节似乎在两种细胞系中不同。一氧化氮（NO）的产生负调节IFN-γ引发的巨噬细胞中IDO活性的表达，从而表明这些细胞中犬尿氨酸和氮能途径之间的串扰。相反，这种由NO引起的IDO活性的下调在小胶质细胞中不发生。还观察到LPS和吡啶甲酸对两种细胞系中IDO表达的差异调节。与先前的发现一起，这些结果表明巨噬细胞和小胶质细胞之间的犬尿氨酸途径酶的表达的调节存在显著差异。
The regulation of the expression of indoleamine 2,3-dioxygenase (IDO) was studied in cloned murine macrophages (MT2) and microglial (N11) cells. Both cell lines express IDO and inducible nitric oxide synthase activity after interferon-gamma (IFN-gamma) stimulation. The regulation of IDO expression appears to differ in the two cell lines. Nitric oxide (NO) production negatively modulates the expression of IDO activity in IFN-gamma-primed macrophages, thereby indicating a cross-talk between the kynurenine and nitridergic pathways in these cells. Conversely, this down-regulation of IDO activity by NO does not occur in microglial cells. A differential regulation of IDO expression in the two cell lines was also observed with LPS and picolinic acid. Together with previous findings, these results indicate the existence of marked differences in the regulation of the expression of the kynurenine pathway enzymes between macrophages and microglial cells.