Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway
Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway
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DOI：
10.1016/s1534-5807(02)00373-8
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发表时间：
2002-12-01
影响因子：
11.8
通讯作者：
Klionsky, DJ
中科院分区：
文献类型：
--
作者：
Shintani, T;Huang, WP;Klionsky, DJ
真核细胞器的正常功能在很大程度上取决于瞬时递送囊泡内货物蛋白的特异性包装。胞质至空泡靶向（Cvt）途径是一种自噬相关的运输途径，其货物蛋白氨肽酶I和α-甘露糖苷酶选择性地从胞质转运到酵母中的溶酶体样空泡。这项研究阐明了在这一途径中货物特异性的分子机制，涉及四个离散的步骤。Cvt 19受体在这一过程中起着核心作用：Cvt 19中的不同结构域识别寡聚货物蛋白，并通过与Cvt 9和Aut 7的相互作用将它们连接到囊泡形成机制。由于自噬是细胞器周转的主要机制，这些结果提供了对细胞内稳态至关重要的生理过程的见解，包括用于溶酶体递送和分解的受损或多余细胞器的特定包装。
The proper functioning of eukaryotic organelles is largely dependent on the specific packaging of cargo proteins within transient delivery vesicles. The cytoplasm to vacuole targeting (Cvt) pathway is an autophagy-related trafficking pathway whose cargo proteins, aminopeptidase I and alpha-mannosidase, are selectively transported from the cytoplasm to the lysosome-like vacuole in yeast. This study elucidates a molecular mechanism for cargo specificity in this pathway involving four discrete steps. The Cvt19 receptor plays a central role in this process: distinct domains in Cvt19 recognize oligomerized cargo proteins and link them to the vesicle formation machinery via interaction with Cvt9 and Aut7. Because autophagy is the primary mechanism for organellar turnover, these results offer insights into physiological processes that are critical in cellular homeostasis, including specific packaging of damaged or superfluous organelles for lysosomal delivery and breakdown.