Oocyte Quality in Mice is Affected by a Mycotoxin-Contaminated Diet
Oocyte Quality in Mice is Affected by a Mycotoxin-Contaminated Diet
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DOI：
10.1002/em.21833
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发表时间：
2014-05-01
影响因子：
2.8
通讯作者：
Sun, Shao-Chen
中科院分区：
文献类型：
--
作者：
Hou, Yan-Jun;Xiong, Bo;Sun, Shao-Chen
真菌毒素，如脱氧雪腐镰刀菌烯醇（DON）、玉米赤霉烯酮（ZEN）和黄曲霉毒素（AF），常见于许多食品中，可能损害动物和人类的生长和生殖效率。我们研究了霉菌毒素污染的饮食对小鼠卵母细胞质量的影响。将被DON（3.875 mg/kg）、ZEN（1，897 g/kg）和AF（806 g/kg）污染的玉米以三种不同水平（0、15和30%w/w）掺入小鼠饮食中。4周后，喂养霉菌毒素的小鼠的卵巢和生殖囊泡卵母细胞指数下降。这些小鼠的卵母细胞表现出较低的发育能力，生殖囊泡破裂和极体排出率降低。胚胎发育能力也表现出类似的模式，大多数胚胎不能发育到桑椹胚阶段。肌动蛋白的表达也减少了卵母细胞皮质和细胞质，这是伴随着肌动蛋白成核因子profilin-1和mDia 1的表达减少。此外，来自喂食霉菌毒素污染饮食的小鼠的大部分卵母细胞表现出异常的纺锤体形态、皮质无颗粒结构域的丧失和异常的线粒体分布，这进一步支持了卵母细胞质量的下降。因此，我们的研究结果表明，霉菌毒素是有毒的小鼠生殖系统，通过影响卵母细胞的质量。Environ.摩尔变异体55：354-362，2014. (c)2013 Wiley Periodicals，Inc.
Mycotoxins, such as deoxynivalenol (DON), zearalenone (ZEN), and aflatoxin (AF), are commonly found in many food commodities and may impair the growth and reproductive efficiency of animals and humans. We investigated the effects of a mycotoxin-contaminated diet on mouse oocyte quality. Maize contaminated with DON (3.875 mg/kg), ZEN (1,897 g/kg), and AF (806 g/kg) was incorporated into a mouse diet at three different levels (0, 15, and 30% w/w). After 4 weeks, ovarian and germinal vesicle oocyte indices decreased in mycotoxin-fed mice. Oocytes from these mice exhibited low developmental competence with reduced germinal vesicle breakdown and polar body extrusion rates. Embryo developmental competence also showed a similar pattern, and the majority of embryos could not develop to the morula stage. Actin expression was also reduced in both the oocyte cortex and cytoplasm, which was accompanied by decreased expression of the actin nucleation factors profilin-1 and mDia1. Moreover, a large percentage of oocytes derived from mice that were fed a mycotoxin-contaminated diet exhibited aberrant spindle morphology, a loss of the cortical granule-free domain, and abnormal mitochondrial distributions, which further supported the decreased oocyte quality. Thus, our results demonstrate that mycotoxins are toxic to the mouse reproductive system by affecting oocyte quality. Environ. Mol. Mutagen. 55:354-362, 2014. (c) 2013 Wiley Periodicals, Inc.