Effects of implant surface microtopography on osteoblast gene expression
Effects of implant surface microtopography on osteoblast gene expression
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DOI：
10.1111/j.1600-0501.2005.01170.x
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发表时间：
2005-12-01
影响因子：
4.3
通讯作者：
Stanford, C
中科院分区：
文献类型：
--
作者：
Masaki, C;Schneider, GB;Stanford, C
目的：在各种种植体表面评价成骨细胞附着和分化的促进作用。然而，不同种植体表面特性对关键成骨因子基因表达的影响尚未完全清楚。目的：本研究的目的是评估钛表面地形效应如何改变骨相关基因和转录因子的表达。方法：将成骨细胞培养在用二氧化钛喷砂处理制备的钛盘上（TiOBlast（TM））或喷砂并用氢氟酸蚀刻（OsseospeedTM），喷砂并蚀刻（SLA-1），或喷砂、蚀刻并用N-2保护冲洗并储存在等渗NaCl（SLA-2）商业纯钛种植体盘中。人骨髓间充质前成骨细胞的高密度培养（HEPM 1486，ATCC）生长72小时，并使用实时PCR定量分析碱性磷酸酶（ALP）、核心结合因子α 1（Cbfa 1）、Osterix、I型胶原、骨钙素和骨唾液蛋白II基因表达。实时PCR显示，相对于所有其他表面，在SLA-2上生长的成骨细胞中ALP基因表达显着增加（P < 0.001）。与SLA-1和SLA-2相比，Osseospeed和TiOBlast表面的Cbfa 1/RUNX-2基因表达显著增加（P < 0.01）。结论：种植体表面性质可能通过影响人骨髓间充质前成骨细胞中骨相关基因和转录因子的表达水平，参与成骨细胞分化的调控。
Aim: The promotion of osteoblast attachment and differentiation has been evaluated on various implant surfaces. However, the effects of different implant surface properties on gene expression of key osteogenic factors are not fully understood.Objective: The objectives of this study were to evaluate how topographical effects on titanium surface alter the expression of bone-related genes and transcription factors.Methods: Osteoblasts were cultured on titanium disks prepared with a titanium dioxide grit blasting (TiOBlast (TM)) or grit blasted and etched with hydrofluoric acid (Osseospeed (TM)), grit blasted and etched (SLA-1), or grit blasted, etched and rinsed with N-2 protection and stored in isotonic NaCl (SLA-2) commercially pure titanium implant discs. High-density cultures of human mesenchymal pre-osteoblastic cells (HEPM 1486, ATCC) were grown for 72 h and real-time PCR used for quantitative analysis of alkaline phosphatase (ALP), core-binding factor alpha 1 (Cbfa1), Osterix, Type I Collagen, Osteocalcin and bone sialoprotein II gene expression.Results: Real-time PCR showed significant (P < 0.001) increases in ALP gene expression in osteoblasts grown on SLA-2, relative to all other surfaces. Cbfa1/RUNX-2 gene expression was significantly (P < 0.01) increased on Osseospeed and TiOBlast surface as compared with SLA-1 and SLA-2 surfaces. The expression of Osterix had a trend similar to that of Cbfa1.Conclusion: In conclusion, implant surface properties may contribute to the regulation of osteoblast differentiation by influencing the level of bone-related genes and transcription factors in human mesenchymal pre-osteoblastic cells.