Role of surface electric charge in red blood cell interactions.
Role of surface electric charge in red blood cell interactions.
复制标题
DOI：
10.1085/jgp.61.5.638
复制
发表时间：
1973-05
影响因子：
3.8
通讯作者：
Chien, S
中科院分区：
文献类型：
--
作者：
Jan, K M;Chien, S
通过比较正常红细胞和神经氨酸酶处理的红细胞的行为，研究了红细胞表面电荷在影响大分子红细胞聚集中的作用。神经氨酸酶可以去除细胞膜上的唾液酸，降低Zeta电位。通过显微镜观察、红细胞沉降率测定和低切粘度测定，对不同相对分子质量的右旋糖苷(Dx 20、Dx 40和Dx 80)的红细胞聚集性进行了定量研究。DX-20不能引起正常RBC的聚集，但能引起神经氨酸酶处理的RBC的大量聚集，神经氨酸酶处理的RBC在DX-40和DX-80也表现出比正常RBC更强的聚集。结合RBC胞间距离随所用右旋糖苷分子大小而变化的电子显微镜观察，本研究表明，RBC的表面电荷抑制了右旋糖苷的聚集，细胞表面之间的静电斥力可能作用于20 nm的距离。
The role of the surface charge of human red blood cells (RBC's) in affecting RBC aggregation by macromolecules was studied by comparing the behavior of normal RBC's with that of RBC's treated with neuraminidase, which removes the sialic acids from the cell membrane and reduces the zeta potential. RBC aggregation in dextrans with different molecular weights (Dx 20, Dx 40, and Dx 80) was quantified by microscopic observation, measurement of erythrocyte sedimentation rate, and determination of low-shear viscosity. Dx 20 did not cause aggregation of normal RBC's, but caused considerable aggregation of neuraminidase-treated RBC's. Neuraminidase-treated RBC's also showed stronger aggregation than normal RBC's in Dx 40 and 80. Together with the electron microscopic findings that the intercellular distance in the RBC rouleaux varies with the molecular size of dextrans used, the present study indicates that the surface charge of RBC's inhibits their aggregation by dextrans and that the electrostatic repulsive force between cell surfaces may operate over a distance of 20 nm.