Dense Intra-adipose Sympathetic Arborizations Are Essential for Cold-Induced Beiging of Mouse White Adipose Tissue
Dense Intra-adipose Sympathetic Arborizations Are Essential for Cold-Induced Beiging of Mouse White Adipose Tissue
复制标题
密集的脂肪内交感神经树枝化对于寒冷诱导的小鼠白色脂肪组织米色化至关重要
DOI：
10.1016/j.cmet.2017.08.016
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发表时间：
2017-10-03
期刊：
影响因子：
29
通讯作者：
Zeng, Wenwen
中科院分区：
文献类型：
--
作者：
Jiang, Haochen;Ding, Xiaofan;Zeng, Wenwen
来自中枢神经系统的传出信号代表白色脂肪组织(WAT)调节的关键层。然而，传出神经信号控制Wat代谢的机制仍有待于更好地了解。在这里，我们利用体积荧光成像技术在单纤维分辨率下可视化小鼠腹股沟神经分支。成像显示交感神经分支的密集网络，这是以前用常规方法没有发现的，交感神经纤维与90%的脂肪细胞紧密对接。我们证明这些交感神经纤维起源于被冷刺激激活的腹腔神经节。交感神经特异性的TrkA受体的缺失或6-羟基多巴胺的药理消融可以取消这些脂肪内的分支，结果是，冷诱导的腹股沟水变黄。此外，我们发现局部交感神经分支通过β-肾上腺素能受体在这一褐变过程中发挥作用。这些发现揭示了传出神经信号与个体脂肪细胞新陈代谢之间的重要联系。
Efferent signals from the central nervous system represent a key layer of regulation of white adipose tissue (WAT). However, the mechanism by which efferent neural signals control WAT metabolism remains to be better understood. Here, we exploit the volume fluorescence-imaging technique to visualize the neural arborizations in mouse inguinal WAT at single-fiber resolution. The imaging reveals a dense network of sympathetic arborizations that had been previously undetected by conventional methods, with sympathetic fibers being in close apposition to > 90% of adipocytes. We demonstrate that these sympathetic fibers originate from the celiac ganglia, which are activated by cold challenge. Sympathetic-specific deletion of TrkA receptor or pharmacologic ablation by 6-hydroxydopamine abolishes these intra-adipose arborizations and, as a result, cold-induced beiging of inguinal WAT. Furthermore, we find that local sympathetic arborizations function through beta-adrenergic receptors in this beiging process. These findings uncover an essential link connecting efferent neural signals with metabolism of individual adipocytes.