Stimulation of expression of a herpes simplex virus DNA-binding protein by two viral functions.
Stimulation of expression of a herpes simplex virus DNA-binding protein by two viral functions.
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DOI：
10.1128/mcb.5.5.957
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发表时间：
1985-05
影响因子：
5.3
通讯作者：
M. P. Quinlan;D. Knipe
中科院分区：
文献类型：
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作者：
M. P. Quinlan;D. Knipe
我们用含有单纯疱疹病毒（HSV） DNA序列的重组质粒转染猴细胞，检测了疱疹病毒蛋白的表达和定位。当编码ICP8的质粒单独导入细胞时，观察到主要的HSV dna结合蛋白ICP8的低水平表达。用编码HSV α（即早期）ICP4和ICP0基因的重组质粒转染ICP8基因后，ICP8的表达显著增加。缺失和亚克隆分析表明，在α基因质粒上编码了两种能够刺激ICP8表达的功能。一个定位在ICP4基因内或附近，一个定位在ICP0基因内或附近。它们的刺激作用是协同的，当引入两个单独的质粒。因此，两种不同的病毒功能可以激活疱疹病毒在转染细胞中的早期基因表达。
We examined the expression and localization of herpesvirus proteins in monkey cells transfected with recombinant plasmids containing herpes simplex virus (HSV) DNA sequences. Low levels of expression of the major HSV DNA-binding protein ICP8 were observed when ICP8-encoding plasmids were introduced into cells alone. ICP8 expression was greatly increased when a recombinant plasmid encoding the HSV alpha (immediate-early) ICP4 and ICP0 genes was transfected with the ICP8 gene. Deletion and subcloning analysis indicated that two separate functions capable of stimulating ICP8 expression were encoded on the alpha gene plasmid. One mapped in or near the ICP4 gene, and one mapped in or near the ICP0 gene. Their stimulatory effects were synergistic when introduced on two separate plasmids. Thus, two separate viral functions can activate herpesvirus early gene expression in transfected cells.