The flavivirus-conserved penta-nucleotide in the 3′ stem-loop of the West Nile virus genome requires a specific sequence and structure for RNA synthesis, but not for viral translation
The flavivirus-conserved penta-nucleotide in the 3′ stem-loop of the West Nile virus genome requires a specific sequence and structure for RNA synthesis, but not for viral translation
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DOI：
10.1016/j.virol.2004.07.022
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发表时间：
2005-01-20
期刊：
影响因子：
3.7
通讯作者：
Shi, PY
中科院分区：
文献类型：
--
作者：
Tilgner, M;Deas, TS;Shi, PY
西尼罗病毒（WN）的报告复制子用于区分3'非翻译区（UTR）在病毒翻译和RNA复制中的功能。复制子的3' UTR的多个区域的缺失不显著影响病毒翻译，但消除RNA复制。系统诱变表明，黄病毒保守的五核苷酸（5 '-CACAG-3'位于基因组3'茎环的顶部）需要特定的序列和结构用于WN RNA合成，但不用于病毒翻译。(i)碱基对结构和五核苷酸第一位的序列对RNA复制至关重要。(ii)第2、3和5位的保守核苷酸对RNA合成是必需的，但第4位的保守核苷酸对RNA合成是必需的。(iii)紧邻五核苷酸下游的核苷酸U（其在黄病毒属中部分保守）对于病毒复制不是必需的。爱思唯尔公司出版
A reporting replicon of West Nile virus (WN) was used to distinguish between the function of the 3' untranslated region (UTR) in viral translation and RNA replication. Deletions of various regions of the 3' UTR of the replicon did not significantly affect viral translation, but abolished RNA replication. A systematic mutagenesis showed that the flavivirus-conserved penta-nucleotide (5'-CACAG-3' located at the top of the 3' stem-loop of the genome) requires a specific sequence and structure for WN RNA synthesis, but not for viral translation. (i) Basepair structure and sequence at the 1st position of the penta-nucleotide are critical for RNA replication. (ii) The conserved nucleotides at the 2nd, 3rd, and 5th positions, but not at the 4th position of the penta-nucleotide, are essential for RNA synthesis. (iii) The nucleotide U (which is partially conserved in the genus Flavivirus) immediately downstream of the penta-nucleotide is not essential for viral replication. Published by Elsevier Inc.