Time-lapse imaging of Arabidopsis leaf development shows dynamic patterns of procambium formation
Time-lapse imaging of Arabidopsis leaf development shows dynamic patterns of procambium formation
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DOI：
10.1111/j.1469-8137.2007.02193.x
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发表时间：
2007-01-01
期刊：
影响因子：
9.4
通讯作者：
Scarpella, Enrico
中科院分区：
文献类型：
--
作者：
Sawchuk, Megan G.;Head, Philip;Scarpella, Enrico
叶脉形成的基本原理长期以来一直引起发育生物学家的兴趣。在叶片中，维管前体原形成层细胞网络通过解剖学上不显眼的前形成层细胞的选择从表面上均匀的表皮下组织中产生。对原形成层形成动力学的理解一直受到在体内观察这一过程的困难的阻碍。在这里，我们提出了一种实时成像技术，允许视觉访问复杂的事件发生在发展叶片。我们将这种方法与拟南芥（拟南芥）的阶段特异性荧光标记相结合，以观察在未受干扰的发育过程中以及在静脉个体发育的明确扰动下，原形成层前链形成和原形成层分化。在所有实验条件下，我们观察到原形成层前链的延伸、终止和融合，以及原形成层沿整个单个静脉分化的同时开始。我们的研究结果强烈表明，原形成层前链形成的进行性和原形成层分化的同时性代表了静脉形成机制的内在特性。
The principles underlying the formation of leaf veins have long intrigued developmental biologists. In leaves, networks of vascular precursor procambial cells emerge from seemingly homogeneous subepidermal tissue through the selection of anatomically inconspicuous preprocambial cells. Understanding dynamics of procambium formation has been hampered by the difficulty of observing the process in vivo.Here we present a live-imaging technique that allows visual access to complex events occurring in developing leaves. We combined this method with stage-specific fluorescent markers in Arabidopsis (Arabidopsis thaliana) to visualize preprocambial strand formation and procambium differentiation during the undisturbed course of development and upon defined perturbations of vein ontogeny.Under all experimental conditions, we observed extension, termination and fusion of preprocambial strands and simultaneous initiation of procambium differentiation along entire individual veins.Our findings strongly suggest that progressiveness of preprocambial strand formation and simultaneity of procambium differentiation represent inherent properties of the mechanism underlying vein formation.