Examining the properties and therapeutic potential of glial restricted precursors in spinal cord injury.
Examining the properties and therapeutic potential of glial restricted precursors in spinal cord injury.
复制标题
检查脊髓损伤中神经胶质限制前体的特性和治疗潜力。
DOI：
10.4103/1673-5374.180725
复制
发表时间：
2016-04
影响因子：
6.1
通讯作者：
Fischer I
中科院分区：
文献类型：
--
作者：
Hayakawa K;Haas C;Fischer I
在脊髓损伤后，胶质限制前体(GRPs)和未成熟的星形胶质细胞提供了调节损伤脊髓的炎症环境和促进宿主轴突再生的潜力。然而，临床应用细胞疗法修复脊髓损伤需要严格的质量保证方案，以便大规模生产和保存，这需要长期的体外扩增。重要的是，这样的过程有可能改变这些细胞的表型和功能特性，从而具有治疗潜力。此外，细胞疗法的临床应用可能受到损伤脊髓炎症微环境的限制，从而改变移植细胞的表型和功能特性。这份报告模拟了玻璃钢的大规模生产过程，并展示了玻璃钢经过长期体外培养后的允许性质。此外，我们定义了在炎症因子存在的情况下GRP的表型和功能特性，并呼吁注意移植细胞微环境的重要性，强调调节损伤脊髓环境的重要性。
In the aftermath of spinal cord injury, glial restricted precursors (GRPs) and immature astrocytes offer the potential to modulate the inflammatory environment of the injured spinal cord and promote host axon regeneration. Nevertheless clinical application of cellular therapy for the repair of spinal cord injury requires strict quality-assured protocols for large-scale production and preservation that necessitates long-term in vitro expansion. Importantly, such processes have the potential to alter the phenotypic and functional properties and thus therapeutic potential of these cells. Furthermore, clinical use of cellular therapies may be limited by the inflammatory microenvironment of the injured spinal cord, altering the phenotypic and functional properties of grafted cells. This report simulates the process of large-scale GRP production and demonstrates the permissive properties of GRP following long-term in vitro culture. Furthermore, we defined the phenotypic and functional properties of GRP in the presence of inflammatory factors, and call attention to the importance of the microenvironment of grafted cells, underscoring the importance of modulating the environment of the injured spinal cord.