Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets
Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets
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DOI：
10.1172/jci36209
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发表时间：
2008-11-01
影响因子：
15.9
通讯作者：
Powers, Alvin C.
中科院分区：
文献类型：
--
作者：
Nyman, Lara R.;Wells, K. Sam;Powers, Alvin C.
胰岛是高度血管化的微器官，在血糖稳态调节中起关键作用。内分泌细胞类型在胰岛中的特定排列表明胰岛内形态和功能之间的耦合。在这里，我们建立了一个线扫描共聚焦显微镜的方法来检查血流量和胰岛细胞类型之间的关系，通过实时在体内成像的胰岛内血流在小鼠的安排。这些数据用于重建胰岛的体内3D结构和整个胰岛血管床的时间分辨血流模式。结果揭示了小鼠胰岛中的两种主要血流模式：从内到外，血液在非β细胞的胰岛周边之前灌注β细胞的核心，以及从上到下，血液从一侧灌注胰岛到另一侧，而不管细胞类型如何。我们的方法包括毫秒时间分辨率和亚微米空间分辨率，允许实时成像的胰岛血流在活的小鼠，这还没有达到我们的知识，通过其他方法。
The pancreatic islets of Langerhans are highly vascularized micro-organs that play a key role in the regulation of blood glucose homeostasis. The specific arrangement of endocrine cell types in islets suggests a coupling between morphology and function within the islet. Here, we established a line-scanning confocal microscopy approach to examine the relationship between blood flow and islet cell type arrangement by real-time in vivo imaging of intra-islet blood flow in mice. These data were used to reconstruct the in vivo 3D architecture of the islet and time-resolved blood flow patterns throughout the islet vascular bed. The results revealed 2 predominant blood flow patterns in mouse islets: inner-to-outer, in which blood perfuses the core of beta cells before the islet perimeter of non-beta cells, and top-to-bottom, in which blood perfuses the islet from one side to the other regardless of cell type. Our approach included both millisecond temporal resolution and submicron spatial resolution, allowing for real-time imaging of islet blood flow within the living mouse, which has not to our knowledge been attainable by other methods.