Strongyloides stercoralis recombinant NIE antigen shares epitope with recombinant Ves v 5 and Pol a 5 allergens of insects
Strongyloides stercoralis recombinant NIE antigen shares epitope with recombinant Ves v 5 and Pol a 5 allergens of insects
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DOI：
10.4269/ajtmh.2005.72.549
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发表时间：
2005-05-01
影响因子：
3.3
通讯作者：
Neva, FA
中科院分区：
文献类型：
--
作者：
Ravi, V;King, TP;Neva, FA
一种新的用于人类类圆线虫感染免疫诊断的重组蛋白（NIE）与抗原5昆虫毒素变应原有13%~ 18%的氨基酸同源性。而NIE的C-末端片段与Ves v5（黄夹克）和Pol a5（纸蜂）的同源性最高。兔抗NIE多克隆抗体可识别NIE抗原的单一条带以及Pol a 5和Ves v 5抗原的条带，小鼠抗Pol a 5和抗Ves v 5血清可与重组NIE抗原反应。溴化氰消化的NIE的C-末端片段与小鼠抗Ves v 5和Pol a 5抗体以及与兔抗NIE血清反应。虽然IgE和IgG抗体从合并血清类圆线虫感染的患者与Pol a 5和Ves v 5重组抗原的免疫印迹反应，无论是抗原抑制人IgG反应与NIE抗原的竞争性酶联免疫吸附试验。
A new recombinant protein (NIE) for immunodiagnosis Of human Strongyloides infection has 13% to 18% amino acid identify with antigen 5 insect venom allergen. but the C-terminal segment of NIE showed highest identify with Ves v 5 (yellow jacket) and Pol a 5 (paper wasp). A rabbit polyclonal anti-NIE antibody identified a single band of NIE antigen as well as hands of Pol a 5 and Ves v 5 antigens, and mouse anti-Pol a 5 and anti-Ves v 5 sera reacted with recombinant NIE antigen by Western blot. A cyanogen bromide-digested C-terminal fragment of NIE was reactive with mouse anti-Ves v 5 and Pol a 5 antibodies as well as with rabbit anti-NIE serum. Although IgE and IgG antibodies from pooled sera from Strongyloides-infected patients reacted with Pol a 5 and Ves v 5 recombinant antigens on immunoblots, neither antigen inhibited human IgG reaction with NIE antigen in a competitive enzyme-linked immunosorbent assay.