Probing excited conformational states of nucleic acids by nitrogen CEST NMR spectroscopy
Probing excited conformational states of nucleic acids by nitrogen CEST NMR spectroscopy
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DOI：
10.1016/j.jmr.2019.106642
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发表时间：
2020-01-01
影响因子：
2.2
通讯作者：
Zhang, Qi
中科院分区：
文献类型：
--
作者：
Zhao, Bo;Baisden, Jared T.;Zhang, Qi
表征低密度和短寿命的激发构象状态对于理解 RNA 功能机制变得越来越重要。 RNA 基态和激发构象之间的相互转换通常涉及碱基配对重排，从而导致氢键网络的变化。在这里，我们提出了两个 N-15 化学交换饱和转移 (CEST) NMR 实验，利用质子化和非质子化氮（关键的氢键供体和受体）来表征 RNA 中的激发构象状态。我们在 B. Cereus 氟化物核糖开关上演示了这些方法，其中 N-15 CEST 图谱补充了 C-13 CEST 图谱，描绘了氟化物核糖开关激发构象状态的配体依赖性变构调节的潜在途径。 (C) 2019 Elsevier Inc. 保留所有权利。
Characterizing low-populated and short-lived excited conformational states has become increasingly important for understanding mechanisms of RNA function. Interconversion between RNA ground and excited conformational states often involves base pairing rearrangements that lead to changes in the hydrogen-bond network. Here, we present two N-15 chemical exchange saturation transfer (CEST) NMR experiments that utilize protonated and non-protonated nitrogens, which are key hydrogen-bond donors and acceptors, for characterizing excited conformational states in RNA. We demonstrated these approaches on the B. Cereus fluoride riboswitch, where N-15 CEST profiles complement C-13 CEST profiles in depicting a potential pathway for ligand-dependent allosteric regulation of the excited conformational state of the fluoride riboswitch. (C) 2019 Elsevier Inc. All rights reserved.