Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2'-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material.
Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2'-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material.
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DOI：
10.1016/j.bios.2004.10.032
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发表时间：
2005-09
影响因子：
12.6
通讯作者：
Zhiai Xu;Zhihui Guo;S. Dong
中科院分区：
文献类型：
--
作者：
Zhiai Xu;Zhihui Guo;S. Dong
An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2′-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol–gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol–gel film to provide the microenvironment for retaining the activity of enzyme and immobilize tris(2,2′-bipyridyl)ruthenium (II). The design was simpler than the previous two-layer format. The experimental conditions, such as scan rate, pH and concentration of the cofactor were investigated. The intensity of electrogenerated chemiluminescence increased linearly with ethanol concentration from 2.5×10−5to 5.0×10−2M and detection limit was 1.0×10−5M. The prepared biosensor exhibited high sensitivity, wide linear range and good stability.
