Host cell traversal is important for progression of the malaria parasite through the dermis to the liver
Host cell traversal is important for progression of the malaria parasite through the dermis to the liver
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DOI：
10.1016/j.chom.2007.12.007
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发表时间：
2008-02-01
影响因子：
30.3
通讯作者：
Menard, Robert
中科院分区：
文献类型：
--
作者：
Amino, Rogerio;Giovannini, Donatella;Menard, Robert
疟疾子孢子，即蚊子传播的寄生虫阶段，被递送到真皮中并在肝脏中分化。游动子孢子可以通过破坏质膜并穿过质膜迁移（称为细胞穿越），或通过形成寄生虫-细胞连接并定居在细胞内空泡内（称为细胞感染）来侵入宿主细胞。在体内观察到的子孢子的肝细胞的迁移被认为激活子孢子以感染最终的肝细胞。在这里，使用伯氏疟原虫，我们表明，细胞穿越是重要的，在宿主真皮防止子孢子破坏吞噬细胞和逮捕非吞噬细胞。我们还表明，细胞感染是一个被掩盖的途径，而不是激活，通过细胞遍历。我们建议，子孢子的细胞穿越活动必须打开进展到肝实质，在那里它必须关闭感染的最终肝细胞。
The malaria sporozoite, the parasite stage transmitted by the mosquito, is delivered into the dermis and differentiates in the liver. Motile sporozoites can invade host cells by disrupting their plasma membrane and migrating through them (termed cell traversal), or by forming a parasite-cell junction and settling inside an intracellular vacuole (termed cell infection). Traversal of liver cells, observed for sporozoites in vivo, is thought to activate the sporozoite for infection of a final hepatocyte. Here, using Plasmodium berghei, we show that cell traversal is important in the host dermis for preventing sporozoite destruction by phagocytes and arrest by nonphagocytic cells. We also show that cell infection is a pathway that is masked, rather than activated, by cell traversal. We propose that the cell traversal activity of the sporozoite must be turned on for progression to the liver parenchyma, where it must be switched off for infection of a final hepatocyte.