FGF-2 is sufficient to isolate progenitors found in the adult mammalian spinal cord
FGF-2 is sufficient to isolate progenitors found in the adult mammalian spinal cord
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DOI：
10.1006/exnr.1997.6697
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发表时间：
1997-12-01
影响因子：
5.3
通讯作者：
Gage, FH
中科院分区：
文献类型：
--
作者：
Shihabuddin, LS;Ray, J;Gage, FH
成年大鼠脑含有祖细胞，其可响应于FGF-2在体外被诱导增殖。在本研究中，我们探索了是否可以从成年大鼠脊髓的不同水平（颈、胸、腰和骶）培养类似的祖细胞，以及它们是否产生神经元和神经胶质以及脊髓特异性神经元（例如，运动神经元）。显微解剖成年大鼠脊髓（>3月龄）的颈、胸、腰和骶区域，分离神经祖细胞并在含有FGF-2（20 ng/ml）的无血清培养基中通过多次传代培养。虽然所有区域都产生了快速增殖的细胞，但培养物本质上是异质的，并且细胞形态在给定区域内以及区域之间存在差异。来自脊髓所有区域的细胞的百分比分化成显示神经元、星形胶质细胞和少突胶质细胞谱系的抗原特性的细胞;然而，来自所有区域的大多数细胞表达未成熟增殖祖细胞标记波形蛋白。在已建立的多次传代培养中，一些大的神经元样细胞表达p75 NGFr免疫反应性，不表达GFAP。这些细胞可能是运动神经元。这些结果表明，FGF-2对来自成年大鼠脊髓的祖细胞具有促有丝分裂作用，所述祖细胞具有产生神经胶质和神经元（包括运动神经元）的潜力。(C)北京：科学出版社.
The adult rat brain contains progenitor cells that can be induced to proliferate in vitro in response to FGF-2. In the present study we explored whether similar progenitor cells can be cultured from different levels (cervical, thoracic, lumbar, and sacral) of adult rat spinal cord and whether they give rise to neurons and glia as well as spinal cord-specific neurons (e.g., motoneurons). Cervical, thoracic, lumbar, and sacral areas of adult rat spinal cord (>3 months old) were microdissected and neural progenitors were isolated and cultured in serum-free medium containing FGF-2 (20 ng/ml) through multiple passages. Although all areas generated rapidly proliferating cells, the cultures were heterogeneous in nature and cell morphology varied within a given area as well as between areas. A percentage of cells from all areas of the spinal cord differentiate into cells displaying antigenic properties of neuronal, astroglial, and oligodendroglial lineages; however, the majority of cells from all regions expressed the immature proliferating progenitor marker vimentin. In established multipassage cultures, a few large, neuron-like cells expressed immunoreactivity for p75NGFr and did not express GFAP. These cells may be motoneurons. These results demonstrate that FGF-2 is mitogenic for progenitor cells from adult rat spinal cord that have the potential to give rise to glia and neurons including motoneurons. (C) 1997 Academic Press.