Novel Promoter Sequence Required for Inductive Expression of the Aspergillus nidulans Endoglucanase Gene eglA
Novel Promoter Sequence Required for Inductive Expression of the Aspergillus nidulans Endoglucanase Gene eglA
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DOI：
10.1271/bbb.70278
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发表时间：
2008-02
期刊：
影响因子：
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通讯作者：
Yoshikazu Endo;Misako Yokoyama;Munenori Morimoto;K. Shirai;G. Chikamatsu;N. Kato;N. Tsukagoshi;Masashi Kato;Tetsuo Kobayashi
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文献类型：
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作者：
Yoshikazu Endo;Misako Yokoyama;Munenori Morimoto;K. Shirai;G. Chikamatsu;N. Kato;N. Tsukagoshi;Masashi Kato;Tetsuo Kobayashi
构巢曲霉中编码主要内切葡聚糖酶EGA的eglA基因的表达由纤维素和纤维二糖诱导，但不由木糖诱导。这表明诱导独立于 XlnR，XlnR 是曲霉中木聚糖分解和纤维素分解基因的转录激活剂。对eglA启动子进行突变分析，以鉴定负责XlnR独立诱导的新顺式元件。跨越-153至-138的区域（CCGTACCTTTTTAGGA），称为CeRE（纤维素响应元件），被发现是eglA基因诱导表达所必需的上游激活元件。
Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning −153 to −138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.