Investigating the use of bacteriophages as a new decolonization strategy for intestinal carriage of CTX-M-15-producing ST131 Escherichia coli: An in vitro continuous culture system model
Investigating the use of bacteriophages as a new decolonization strategy for intestinal carriage of CTX-M-15-producing ST131 Escherichia coli: An in vitro continuous culture system model
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DOI：
10.1016/j.jgar.2020.05.018
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发表时间：
2020-09-01
影响因子：
4.6
通讯作者：
Endimiani, Andrea
中科院分区：
文献类型：
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作者：
Bernasconi, Odette J.;Campos-Madueno, Edgar, I;Endimiani, Andrea
目的：我们研究了使用噬菌体作为一种策略，以去殖民化肠道载体的多重耐药Escherichia coli.Methods：发酵罐作为一个连续的培养系统，为48 h。从志愿者中获得的两个不同粪便池（研究I和II）加标产CTX-M-15的ST 131 E。大肠杆菌（菌株4901.28）对噬菌体敏感，并在接种后2、6和10小时用三个剂量的INTESTI噬菌体混合物进行攻击。通过使用Nanopore和Illumina平台进行微量稀释板、斑点试验、rep-PCR和全基因组测序（包括cgMLST和单核苷酸变异分析）来进行细菌分型。
Objectives: We investigated the use of bacteriophages as a strategy to decolonize intestinal carriers of multidrug-resistant Escherichia coli.Methods: A fermentor was used as a continuous culture system for 48 h. Two different pools of faeces (studies I and II) obtained from volunteers were spiked with a CTX-M-15-producing ST131 E. coli (strain 4901.28) susceptible to bacteriophages and challenged with three doses of INTESTI Bacteriophage cocktail administered at 2, 6 and 10 h after the inoculum. Bacterial typing was performed by implementing microdilution panels, spot test, rep-PCR and whole-genome sequencing (including cgMLST and single-nucleotide variant analysis) obtained using Nanopore and Illumina platforms.Results: In study I, bacteriophages decreased the numbers of 4901.28 dramatically (