FUCOSE-CONTAINING ANTIGENS IN NORMAL AND NEOPLASTIC HUMAN GASTRIC-MUCOSA - A COMPARATIVE-STUDY USING LECTIN HISTOCHEMISTRY AND BLOOD-GROUP IMMUNOHISTOCHEMISTRY
FUCOSE-CONTAINING ANTIGENS IN NORMAL AND NEOPLASTIC HUMAN GASTRIC-MUCOSA - A COMPARATIVE-STUDY USING LECTIN HISTOCHEMISTRY AND BLOOD-GROUP IMMUNOHISTOCHEMISTRY
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DOI：
10.1002/path.1711520104
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发表时间：
1987-05-01
影响因子：
7.3
通讯作者：
MACARTNEY, JC
中科院分区：
文献类型：
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作者：
MACARTNEY, JC
组织化学结合正常和肿瘤的人胃粘膜的两个凝集素-过氧化物酶共轭物，这是特定的岩藻糖含糖复合物的描述。凝集素是荆豆（UEA 1）和百脉根（LTA）。结果与ABO和分泌状态和免疫组化证明的1型抗原（Lewisa）和两个2型抗原（X和H）使用单克隆抗体进行比较。UEA 1和LTA与正常胃粘膜表面粘液细胞的结合仅见于分泌型，但与ABO血型无关。在胃癌中凝集素结合减少。UEA 1结合与H 2型抗原和分泌活性的免疫组化显示之间存在关系。相反，LTA染色与H 2型和莱亚抗原相关，但与分泌状态无关。X抗原仅在少量中可证实。尽管这两种凝集素的结合模式存在细微差异，但针对确定寡糖的单克隆抗体的免疫组织化学研究提供了更多信息。研究结果与岩藻糖基和唾液酸转移酶之间的竞争性相互作用发生在胃恶性肿瘤，并导致异常血型相关抗原的表达的假设是一致的。
The histochemical binding to normal and neoplastic human gastric mucosa of two lectin-peroxidase conjugates which are specific for fucose-containing glycoconjugates is described. The lectins are Ulex europaeus (UEA1) and Lotus tetragonolobus (LTA). Results are compared with ABO and secretor status and the immunohistochemical demonstration of a Type 1 antigen (Lewisa) and two Type 2 antigens (X and H) using monoclonal antibodies. Binding of UEA 1 and LTA to surface mucus cells in normal gastric mucosa is only seen in secretors but is independent of ABO status. In gastric carcinomas lectin binding is reduced. There is a relationship between UEA1 binding and the immunohistochemical demonstration of H Type 2 antigen and secretor activity. In contrast LTA staining is associated with both H Type 2 and Lea antigen but not with secretor status. X antigen is only demonstrable in small amounts. Despite subtle differences in the binding patterns of the two lectins, immunohistochemical studies with monoclonal antibodies against defined oligosaccharides provide greater information. The results of the study are consistent with the hypothesis that competitive interaction between fucosyl and sialyl transferases occurs in gastric malignancy and leads to the expression of abnormal blood group-related antigens.