SUBCELLULAR-LOCALIZATION OF TAU-MESSENGER RNA IN DIFFERENTIATING NEURONAL CELL-CULTURE - IMPLICATIONS FOR NEURONAL POLARITY
SUBCELLULAR-LOCALIZATION OF TAU-MESSENGER RNA IN DIFFERENTIATING NEURONAL CELL-CULTURE - IMPLICATIONS FOR NEURONAL POLARITY
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DOI：
10.1016/0896-6273(93)90165-n
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发表时间：
1993-04-01
期刊：
影响因子：
16.2
通讯作者：
GINZBURG, I
中科院分区：
文献类型：
--
作者：
LITMAN, P;BARG, J;GINZBURG, I
来自胎鼠全脑的原代神经元细胞培养物用于分析tau mRNA的亚细胞定位，采用原位杂交的非同位素检测。该培养物表现出先前描述的体内神经元细胞的发育分化模式;即，从未成熟到成熟的tau同种型的转变以及tau分离到轴突中。我们的研究结果表明，不同于微管蛋白mRNA，这是局限于细胞体，或MAP 2 mRNA，延伸到树突，tau mRNA被观察到进入轴突的近端部分。tau mRNA的这种排序可能解释了tau蛋白如何选择性地递送到轴突，并且可能对神经元极性的发展具有重要影响。
A primary neuronal cell culture derived from whole brains of fetal rats was used to analyze the subcellular localization of tau mRNA, employing nonisotopic detection by in situ hybridization. The culture exhibited a developmental differentiation pattern previously described for neuronal cells in vivo; i.e., a transition from immature to mature tau isoforms as well as segregation of tau into the axons. Our results demonstrate that unlike tubulin mRNA, which is confined to cell bodies, or MAP2 mRNA, which extends into dendrites, tau mRNA was observed to enter the proximal portion of the axon. This sorting of tau mRNA might explain how the tau protein could be selectively delivered to the axon and could have important implications for the development of neuronal polarity.