Drought-induced disturbance of carbohydrate metabolism in anthers and male abortion of two Gossypium hirsutum cultivars differing in drought tolerance
Drought-induced disturbance of carbohydrate metabolism in anthers and male abortion of two Gossypium hirsutum cultivars differing in drought tolerance
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干旱引起的花药碳水化合物代谢紊乱和两个耐旱性不同的陆地棉品种的雄性败育
DOI：
10.1007/s00299-019-02483-1
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发表时间：
2019-11-04
影响因子：
6.2
通讯作者：
Zhou, Zhiguo
中科院分区：
文献类型：
--
作者：
Hu, Wei;Huang, Yanjun;Zhou, Zhiguo
干旱胁迫下棉花花粉败育与花药碳水化合物代谢的变化密切相关，干旱敏感品种的花粉败育率高于耐旱品种。干旱胁迫下的棉花生殖失败与雄性育性的改变有着内在的联系，然而，干旱胁迫对棉花雄性育性影响的研究尚不存在。为此，以两个耐旱性不同的棉花品种为材料，进行了干旱胁迫试验，以研究棉花花粉育性和花药生理。结果表明，干旱胁迫降低花粉育性的两个品种，由于减少花药淀粉和三磷酸腺苷（ATP）的合成。干旱胁迫下花药淀粉含量下降的主要原因是同化物供应能力下降以及ADP-葡萄糖焦磷酸化酶和可溶性淀粉合成酶活性降低。蔗糖合成酶和酸性转化酶活性的降低是干旱胁迫下花药中蔗糖含量高于正常供水花药的主要原因，而蔗糖合成酶基因（GhSusA、GhSusB、GhSusD）和酸性转化酶基因（GhINV 1、GhINV 2）表达的降低则加剧了干旱胁迫下花药中蔗糖含量的变化趋势。然而，尽管干旱胁迫下花药中蔗糖的降解受到限制，但干旱胁迫下花药中葡萄糖的含量却高于充分供水的花药，这可能是由于干旱胁迫下花药ATP含量降低，导致花药呼吸下调所致。与耐旱品种相比，干旱胁迫对干旱敏感品种花粉育性的抑制作用更强，这是由于干旱胁迫使花粉中淀粉和ATP含量下降幅度更大。
Cotton pollen abortion, under drought stress, was closely associated with changes in anther carbohydrate metabolism, and pollen abortion rate due to drought was higher in drought-sensitive cultivars than drought-tolerant cultivars. Cotton reproductive failure under drought stress is intrinsically connected with altered male fertility, however, studies investigating the effect of drought stress on cotton male fertility are nonexistent. Thus, a drought stress experiment was conducted with two cotton cultivars, differing in drought tolerance, to study pollen fertility and anthers' physiology. Results indicated that drought stress reduced pollen fertility of both cultivars due to decreases in anther starch and adenosine triphosphate (ATP) synthesis. Lower assimilate supply capacity in conjunction with impaired activities of ADP-glucose pyrophosphorylase and soluble starch synthase were the main reasons for the decreased starch levels in drought-stressed anthers. The decreased activities of sucrose synthetase and acid invertase were responsible for the higher sucrose level in drought-stressed anthers than well-watered anthers and the changing trend of sucrose was intensified by the decreased expressions of sucrose synthase genes (GhSusA, GhSusB, GhSusD) and acid invertase genes (GhINV1, GhINV2). However, despite sucrose degradation being limited in drought-stressed anthers, glucose level was higher in droughted anthers than well-watered ones, and that might be attributed to the down-regulated respiration since decreased anther ATP levels were detected in drought-stressed plants. Furthermore, compared to the drought-tolerant cultivar, pollen fertility was more suppressed by drought stress for the drought-sensitive cultivar, and that was attributed to the larger decrease in starch and ATP contents.