Electrical coupling in sustentacular cells of the mouse olfactory epithelium.
Electrical coupling in sustentacular cells of the mouse olfactory epithelium.
复制标题
小鼠嗅觉上皮支持细胞的电耦合。
DOI：
10.1152/jn.01299.2004
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Lucero,MaryT
中科院分区：
文献类型：
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作者：
Vogalis,Fivos;Hegg,ColleenC;Lucero,MaryT
支撑细胞（SCs）排列在嗅觉上皮（OE）的顶端表面，为嗅觉受体神经元提供营养、代谢和机械支持。形态学研究表明，SCs具有间隙连接，尽管缺乏哺乳动物SCs中间隙连接通信的生理证据。在本研究中，我们研究了位于新生儿（P0-P4）小鼠OE组织切片中的SCs之间是否存在偶联。利用SCs的全细胞和细胞贴附贴片记录，我们证明SCs通过300 MΩ量级的结电阻电偶联。在全细胞记录条件下，添加到移液液中的Alexa 488未能显示SCs之间的染料偶联。电耦合是由sc记录的电容电流的双指数衰减和p2y受体激活电流的钟形电压依赖性推断出来的，两者都被18β-甘草次酸（20-50 μM）消除，这是一种间隙连接阻滞剂。这些数据为sc之间的功能偶联提供了强有力的证据，并讨论了其生理重要性。
Sustentacular cells (SCs) line the apical surface of the olfactory epithelium (OE) and provide trophic, metabolic, and mechanical support for olfactory receptor neurons. Morphological studies have suggested that SCs possess gap junctions, although physiological evidence for gap junctional communication in mammalian SCs is lacking. In the present study we investigated whether coupling exists between SCs situated in tissue slices of OE from neonatal (P0–P4) mice. Using whole cell and cell-attached patch recordings from SCs, we demonstrate that SCs are electrically coupled by junctional resistances on the order of 300 MΩ. Under whole cell recording conditions, Alexa 488 added to the pipette solution failed to reveal dye coupling between SCs. Electrical coupling was deduced from the biexponential decay of capacitive currents recorded from SCs and from the bell-shaped voltage dependency of a P2Y-receptor–activated current, both of which were abolished by 18β-glycyrrhetinic acid (20–50 μM), a blocker of gap junctions. These data provide strong evidence for functional coupling between SCs, the physiological importance of which is discussed.