A lentiviral expression system demonstrates that L* protein of Theiler's murine encephalomyelitis virus (TMEV) is essential for virus growth in a murine macrophage-like cell line
A lentiviral expression system demonstrates that L* protein of Theiler's murine encephalomyelitis virus (TMEV) is essential for virus growth in a murine macrophage-like cell line
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DOI：
10.1016/j.virusres.2004.07.009
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发表时间：
2005-03-01
期刊：
影响因子：
5
通讯作者：
Sawada, M
中科院分区：
文献类型：
--
作者：
Himeda, T;Ohara, Y;Sawada, M
泰特氏小鼠脑脊髓炎病毒的DA亚群毒株可合成L蛋白，该蛋白与另一种AUG的多聚蛋白一起被翻译出框外，AUG的下游有13个核苷酸。通过使用突变病毒DAL*-1进行的功能丧失实验，在该实验中，L*AUG突变为ACG，L*蛋白被证明在病毒持续、TMEV诱导的脱髓鞘和病毒在巨噬细胞中的生长中发挥重要作用。在本研究中，我们建立了一个L蛋白表达的巨噬细胞样细胞系，并证实了L蛋白在该细胞系中对病毒生长的重要作用。(C)2004爱思唯尔B.V.保留所有权利。
The DA subgroup strains of Theiter's murine encephalomyelitis virus (TMEV) synthesize L* protein, which is translated out of frame with the polyprotein from an alternative AUG, 13 nucleotides downstream from the authentic polyprotein AUG. By a 'loss of function' experiment using a mutant virus, DAL*-1, in which the L* AUG is mutated to an ACG, L* protein is shown to play an important role in virus persistence, TMEV-induced demyelination, and virus growth in macrophages. In the present study, we established an L* protein-expressed macrophage-like cell line and confirmed the importance of L* protein in virus growth in this cell line. (C) 2004 Elsevier B.V. All rights reserved.