Real-time NASBA for detection of Strawberry vein banding virus
Real-time NASBA for detection of Strawberry vein banding virus
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DOI：
10.1023/b:ejpp.0000015378.27255.12
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发表时间：
2004-02-01
影响因子：
1.8
通讯作者：
Jelkmann, W
中科院分区：
文献类型：
--
作者：
Vasková, D;Spak, J;Jelkmann, W
草莓脉带病毒（SVBV）的检测方法。描述了基于核酸序列的扩增（NASBA）和使用分子信标的实时检测（实时NASBA）。该检测方法与生物索引，目前的SVBV认证方法和新优化的基于PCR的检测方法进行了比较。在草莓指示植物和317场草莓从五个欧洲国家的SVBV分离株的检测性能进行了测试。该检测被证明是SVBV特异性的，对其他常见的蚜虫传播的草莓病毒检测呈阴性。在稀释了100万倍的纯化总RNA制剂中检测到病毒，这相当于1 ng新鲜材料的量。实时NASBA的方法，在这里开发的草莓股票材料的常规诊断提供了一个快速，灵敏和可靠的方法的潜力。
An assay for the detection of Strawberry vein banding virus (SVBV) in Fragaria spp. based on nucleic acid sequence based amplification (NASBA) and real-time detection using molecular beacons (real-time NASBA) is described. This assay was compared both with biological indexing, the current method for certification of SVBV, and a newly optimised PCR-based detection method. Performance of the assay was tested on three SVBV isolates in Fragaria indicator plants and 317 field strawberries from five European countries. The assay was shown to be SVBV-specific, testing negative for other common aphid-borne strawberry viruses. The virus was detected in purified total RNA preparations diluted a millionfold, which is an amount equivalent to similar to1 ng of fresh material. The real-time NASBA method developed here offers the potential of a fast, sensitive and reliable approach for the routine diagnosis of strawberry stock material.