Brunn: An open source laboratory information system for microplates with a graphical plate layout design process
Brunn: An open source laboratory information system for microplates with a graphical plate layout design process
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DOI：
10.1186/1471-2105-12-179
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发表时间：
2011-05-20
影响因子：
3
通讯作者：
Wikberg, Jarl E. S.
中科院分区：
文献类型：
--
作者：
Alvarsson, Jonathan;Andersson, Claes;Wikberg, Jarl E. S.
背景：化合物分析和药物筛选会产生大量数据，并且通常基于微孔板测定。当前用于处理此问题的信息系统主要是商业的、闭源的、昂贵的和重量级的，需要一个灵活的轻量级开放系统来处理板设计、数据验证和准备。 结果：构建了由客户端部分和关系数据库组成的Bioclipse插件。 Bioclipse 内部实现了多步骤板布局点击界面。该系统包含一个数据验证步骤，可以删除异常值，最后提供包含所有相关计算数据（包括剂量反应曲线）的板报告。结论：Brunn 能够处理来自微孔板测定的数据。它可以创建剂量反应曲线并计算 IC50 值。使用此类系统有利于实验室的工作。通过从印版布局设计过程的早期步骤开始，能够重复使用已构建的印版和印版布局，从而节省时间并减少错误源。
Background: Compound profiling and drug screening generates large amounts of data and is generally based on microplate assays. Current information systems used for handling this are mainly commercial, closed source, expensive, and heavyweight and there is a need for a flexible lightweight open system for handling plate design, and validation and preparation of data.Results: A Bioclipse plugin consisting of a client part and a relational database was constructed. A multiple-step plate layout point-and-click interface was implemented inside Bioclipse. The system contains a data validation step, where outliers can be removed, and finally a plate report with all relevant calculated data, including dose-response curves.Conclusions: Brunn is capable of handling the data from microplate assays. It can create dose-response curves and calculate IC50 values. Using a system of this sort facilitates work in the laboratory. Being able to reuse already constructed plates and plate layouts by starting out from an earlier step in the plate layout design process saves time and cuts down on error sources.