Hypochlorous acid is a potent inhibitor of acetylcholinesterase
Hypochlorous acid is a potent inhibitor of acetylcholinesterase
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DOI：
10.1006/taap.2002.9419
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发表时间：
2002-06-15
影响因子：
3.8
通讯作者：
Bast, A
中科院分区：
文献类型：
--
作者：
den Hartog, GJM;Vegt, E;Bast, A
过氧化氢和过氧亚硝酸盐在诱导气道高反应性中的作用已被充分描述。在气道炎症期间形成的另一种反应性物质是次氯酸（HOCl）。在本研究中，HOCl对气道胆碱能神经支配的影响进行了研究。观察到HOCl能够增加电刺激气管平滑肌的基础张力。发现HOCl抑制纯化的乙酰胆碱酯酶，IC 50值为0.66 μ M。乙酰胆碱酯酶活性降低可使乙酰胆碱蓄积，增加气道肌张力。HOCl对离体器官和酶制剂的影响可以用含巯基的化合物如还原型谷胱甘肽和N-乙酰半胱氨酸来排除。本研究结果表明，HOCl可以作为乙酰胆碱酯酶的抑制剂。这一发现的诱导气道高反应性的影响进行了讨论。(C)2002 Elsevier Science（美国）。
The role of hydrogen peroxide and peroxynitrite in the induction of airway hyperreactivity has been well described. Another reactive species which is formed during airway inflammation is hypochlorous acid (HOCl). In the present investigation the effect of HOCl on cholinergic innervation of the airway was investigated. It was observed that HOCl was capable of increasing the basal tension of electrically stimulated tracheal smooth muscle. It was found that HOCl inhibits purified acetylcholinesterase with an IC50 value of 0.66 muM. Decreased acetylcholinesterase activity could allow accumulation of acetylcholine and increased airway muscle tension. The effects of HOCl on the isolated organ and the enzyme preparation could be precluded with thiol group-containing compounds such as reduced glutathione and N-acetylcysteine. The present findings indicate that HOCl can act as inhibitor of acetylcholinesterase. The implications of this finding for the induction of airway hyperreactivity are discussed. (C) 2002 Elsevier Science (USA).