Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells.
Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells.
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大鼠脑 Na,K-ATP 酶 β 链基因：一级结构、组织特异性表达和哇巴因耐药 HeLa C 细胞中的扩增。
DOI：
10.1128/mcb.6.11.3884-3890.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
Levenson,R
中科院分区：
文献类型：
--
作者：
Mercer,RW;Schneider,JW;Savitz,A;Emanuel,J;BenzJr,EJ;Levenson,R
我们从cDNA中推导出大鼠脑Na,K-ATP酶β亚基的完整氨基酸序列。大鼠脑β-亚基与人和鱼雷β-亚基多肽表现出高度的一级序列和二级结构同源性。对大鼠组织 RNA 的分析表明，β 亚基基因编码四种不同的 mRNA 种类，它们以组织特异性方式表达。在哇巴因耐药的 HeLa C+ 细胞中，β 亚基 DNA 序列被扩增（约 20 倍），并且 β 亚基 mRNA 相对于亲代 HeLa 细胞中的水平过量产生。这些结果表明，β-亚基在Na,K-ATP酶结构功能和细胞对强心苷抵抗的机制中发挥着重要作用。
We deduced the complete amino acid sequence of the rat brain Na,K-ATPase β-subunit from cDNA. The rat brain β-subunit exhibits a high degree of primary sequence and secondary structural homology with the human andTorpedoβ-subunit polypeptides. Analysis of rat tissue RNA reveals that the β-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+cells, β-subunit DNA sequences are amplified (~20-fold) and β-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the β-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.