Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia
Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia
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DOI：
10.1038/nprot.2009.185
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发表时间：
2009-01-01
期刊：
影响因子：
14.8
通讯作者：
Limbourg, Florian P.
中科院分区：
文献类型：
--
作者：
Limbourg, Anne;Korff, Thomas;Limbourg, Florian P.
成年生物体中的血管生长涉及以下两个基本过程：血管生成，毛细血管网络的增殖和延伸;以及动脉生成，功能性动脉的生长。我们提供了一个议定书的评价出生后的动脉生成和血管生成的小鼠模型后肢缺血。在特定部位手术结扎股动脉可触发小的、预先存在的侧支动脉向近端的功能性管道血管和远端的缺血性血管生成。血管对后肢缺血的反应可以通过基于激光多普勒的灌注测量、动脉生成和血管生成的组织学定量或肢体肌肉中动脉的整体可视化来容易地评估。根据实验设计，该方案需要4至29天才能完成;然而，每只小鼠的净工作时间约为2天。同时和特定的分析出生后血管生成和动脉生成在同一动物是一个独特的功能的协议。
Blood vessel growth in adult organisms involves the following two fundamental processes: angiogenesis, the proliferation and extension of capillary networks; and arteriogenesis, the growth of functional arteries. We provide a protocol for the evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia. Surgical ligation of the femoral artery at a specific site triggers arteriogenesis of small, pre-existing collateral arteries into functional conduit vessels proximally and ischemic angiogenesis distally. The vascular response to hind-limb ischemia can be readily evaluated by laser Doppler-based perfusion measurements, histological quantification of arteriogenesis and angiogenesis or whole-mount visualization of arteries in limb muscles. Depending on the experimental design, the protocol takes between 4 and 29 d to complete; however, the net working time is about 2 d per mouse. The concurrent and specific analysis of postnatal angiogenesis and arteriogenesis in the same animal is a unique feature of the protocol.