Further Biochemical Profiling of Hypholoma fasciculare Metabolome Reveals Its Chemogenetic Diversity.
Further Biochemical Profiling of Hypholoma fasciculare Metabolome Reveals Its Chemogenetic Diversity.
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肌瘤肌瘤代谢组的进一步的生化分析揭示了其化学遗传学多样性。
DOI：
10.3389/fbioe.2021.567384
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发表时间：
2021
影响因子：
5.7
通讯作者：
Foster GD
中科院分区：
文献类型：
--
作者：
Al-Salihi SAA;Bull ID;Al-Salhi R;Gates PJ;Salih KSM;Bailey AM;Foster GD
迫切需要具有新颖化学性质的天然产物来应对微生物耐药性的持续增加。由于蘑菇培养真菌具有挑战性的培养制备和遗传难解性，文献中并未充分利用其作为新型抗生素的来源。然而，现代真菌分子和合成生物学工具对探索蘑菇真菌寻找新型治疗剂重新产生了兴趣。本研究的目的是研究九种担子菌的次生代谢产物，筛选其生物和化学特性，然后研究与其生产相关的遗传途径。在所选的九种真菌中，束状丝状菌被证明是一种高度活性的拮抗物种，对三种不同的微生物具有抗菌活性：枯草芽孢杆菌、大肠杆菌和酿酒酵母。通过基因组比较和色谱研究来表征超过 15 个生物合成基因簇，并鉴定出 3,5-二氯甲氧基苯甲酸是一种潜在的抗菌化合物。还预测了该产品的生物合成基因簇。这项研究增强了蘑菇形成真菌作为尚未开发的生物活性天然产物库的潜力。获取基因组数据和基于化学的框架将有助于新型分子的开发和应用，并应用于制药和农化行业。
Natural products with novel chemistry are urgently needed to battle the continued increase in microbial drug resistance. Mushroom-forming fungi are underutilized as a source of novel antibiotics in the literature due to their challenging culture preparation and genetic intractability. However, modern fungal molecular and synthetic biology tools have renewed interest in exploring mushroom fungi for novel therapeutic agents. The aims of this study were to investigate the secondary metabolites of nine basidiomycetes, screen their biological and chemical properties, and then investigate the genetic pathways associated with their production. Of the nine fungi selected, Hypholoma fasciculare was revealed to be a highly active antagonistic species, with antimicrobial activity against three different microorganisms: Bacillus subtilis, Escherichia coli, and Saccharomyces cerevisiae. Genomic comparisons and chromatographic studies were employed to characterize more than 15 biosynthetic gene clusters and resulted in the identification of 3,5-dichloromethoxy benzoic acid as a potential antibacterial compound. The biosynthetic gene cluster for this product is also predicted. This study reinforces the potential of mushroom-forming fungi as an underexplored reservoir of bioactive natural products. Access to genomic data, and chemical-based frameworks, will assist the development and application of novel molecules with applications in both the pharmaceutical and agrochemical industries.
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DOI：
10.1074/jbc.m110.165845
发表时间：
2011-03-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Engels B;Heinig U;Grothe T;Stadler M;Jennewein S
通讯作者：
Jennewein S
影响因子：
5.4
作者：
Kallberg, Yvonne;Oppermann, Udo;Persson, Bengt
通讯作者：
Persson, Bengt
影响因子：
--
作者：
Eastwood, Daniel C.;Challen, Michael P.;Burton, Kerry S.
通讯作者：
Burton, Kerry S.
穿越真菌萜烯组。
影响因子：
11.9
作者：
Quin MB;Flynn CM;Schmidt-Dannert C
通讯作者：
Schmidt-Dannert C
影响因子：
3
作者：
Godio, Ramiro P.;Martin, Juan F.
通讯作者：
Martin, Juan F.