Colletotrichum shisoi sp. nov., an anthracnose pathogen of Perilla frutescens in Japan: molecular phylogenetic, morphological and genomic evidence
Colletotrichum shisoi sp. nov., an anthracnose pathogen of Perilla frutescens in Japan: molecular phylogenetic, morphological and genomic evidence
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DOI：
10.1038/s41598-019-50076-5
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发表时间：
2019-09-16
影响因子：
4.6
通讯作者：
Shirasu, K.
中科院分区：
文献类型：
--
作者：
Gan, P.;Tsushima, A.;Shirasu, K.
炭疽菌属（Colletotrichum）真菌是世界上农作物最具破坏性的病原体之一。基于DNA序列数据（ITS、GAPDH、CHS-1、ACT、TUB2）和形态学，我们揭示了侵染油料作物紫苏（俗称紫苏）的炭疽菌菌株代表了一种以前未知的炭疽菌。destructivum species complex，并将其命名为C. shisoi。我们发现C. shisoi似乎能够采用半活体营养的生活方式，其特征在于在P.frutescens上形成活体营养菌丝，随后严重坏死损伤，但与其近亲C. higginsianum也属于C.破坏性物种复合体。C.对shisoi进行了测序、注释，并将其预测的蛋白质组与其他四种炭疽菌进行了比较。预测的C. shisoi和C. Higginsianum共有许多候选效应子，它们是可能导致感染的小的分泌蛋白。有趣的是，C。destructivum物种复合体特异性分泌蛋白显示出增加的多样化选择的证据，这可能与它们的宿主特异性有关。
Species of the fungal genus Colletotrichum are among the most devastating pathogens of agricultural crops in the world. Based on DNA sequence data (ITS, GAPDH, CHS-1, ACT, TUB2) and morphology, we revealed Colletotrichum isolates infecting the oil crop Perilla frutescens, commonly known as shiso, to represent a previously unknown species of the C. destructivum species complex and described it as C. shisoi. We found that C. shisoi appears to be able to adopt a hemibiotrophic lifestyle, characterised by the formation of biotrophic hyphae followed by severe necrotic lesions on P. frutescens, but is less virulent on Arabidopsis, compared to its close relative C. higginsianum which also belongs to the C. destructivum species complex. The genome of C. shisoi was sequenced, annotated and its predicted proteome compared with four other Colletotrichum species. The predicted proteomes of C. shisoi and C. higginsianum, share many candidate effectors, which are small, secreted proteins that may contribute to infection. Interestingly, C. destructivum species complex-specific secreted proteins showed evidence of increased diversifying selection which may be related to their host specificities.