Differential susceptibility of equine and mouse brain microvascular endothelial cells to equine herpesvirus 1 infection
Differential susceptibility of equine and mouse brain microvascular endothelial cells to equine herpesvirus 1 infection
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DOI：
10.1007/s00705-005-0653-3
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发表时间：
2006-04-01
影响因子：
2.7
通讯作者：
Umemura, T
中科院分区：
文献类型：
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作者：
Hasebe, R;Kimura, T;Umemura, T
马疱疹病毒1型(EHV-1)在感染的马的中枢神经系统(CNS)中表现出亲内皮作用。然而，在感染小鼠的中枢神经系统中没有观察到内皮细胞的感染。为了探讨这种亲内皮性差异的基础，我们比较了马脑微血管内皮细胞(EBMECs)和小鼠脑微血管内皮细胞(MBMECs)对EHV-1感染的敏感性。病毒在EBMECs中的生长动力学是典型的完全生产性感染，而在MBMEC中的病毒感染似乎是非生产性的。用抗EHV-1多克隆抗体免疫荧光显微镜观察，在感染的EBMECs中发现病毒抗原，但在感染的MBMECs中未发现病毒抗原。EHV-1即刻早期(IE)、早期(ICP0)和晚期(GB、GD和GK)转录本在感染的EBMEC中均有表达。然而，通过逆转录聚合酶链式反应，在感染的MBMEC中没有检测到这些基因。病毒侵入阶段的电子显微镜检查显示，EBMECs胞浆内的未包被的小泡内有病毒颗粒，而MBMECs的胞浆内无病毒颗粒。这些结果表明，病毒进入是EBMECs和MBMECs对EHV-1感染易感性的重要决定因素。
Equine herpesvirus 1 (EHV-1) shows endotheliotropism in the central nervous system (CNS) of infected horses. However, infection of endothelial cells has not been observed in the CNS of infected mice. To explore the basis for this difference in endotheliotropism, we compared the susceptibility of equine brain microvascular endothelial cells (EBMECs) and mouse brain microvascular endothelial cells (MBMECs) to EHV-1 infection. The kinetics of viral growth in EBMECs was typical of a fully productive infection whereas viral infection in MBMECs seemed to be nonproductive. Immunofluorescence microscopy using anti-EHV-1 polyclonal antibody demonstrated viral antigen in infected EBMECs, but not infected MBMECs. EHV-1 immediate early (IE), early (ICP0), and late (gB, gD and gK) transcripts were expressed in infected EBMECs. However, none of these genes was detected in infected MBMECs by reverse transcription-polymerase chain reaction. Electron microscopic examination at the stage of viral entry showed that viral particles were present within uncoated vesicles in the cytoplasm of EBMECs, but absent from those of MBMECs. These results suggest that viral entry is an important determinant of the susceptibility of EBMECs and MBMECs to EHV-1 infection.