INFLUENCE OF TRYPSIN ON LIPOLYSIS IN HUMAN FAT-CELLS - COMPARISON WITH RAT ADIPOCYTES
INFLUENCE OF TRYPSIN ON LIPOLYSIS IN HUMAN FAT-CELLS - COMPARISON WITH RAT ADIPOCYTES
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DOI：
10.1016/0005-2760(76)90009-6
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
NORDMANN, R
中科院分区：
文献类型：
--
作者：
GIUDICELLI, Y;PROVIN, D;NORDMANN, R
通过用胶原酶加胰蛋白酶消化脂肪组织获得经胰蛋白酶处理的人和大鼠脂肪细胞，并将它们对胰岛素、儿茶酚胺和二丁酰环[c]AMP的脂肪分解反应与仅用胶原酶分离的人和大鼠脂肪细胞的脂肪分解反应进行比较。在人和大鼠的脂肪细胞中，细胞内乳酸脱氢酶含量和胰蛋白酶消化后甘油的基础释放没有发生显着变化。在大鼠脂肪细胞中，胰蛋白酶消除了胰岛素的抗脂解作用，但维持了对肾上腺素、去甲肾上腺素和异丙肾上腺素的正常脂解反应。相反，在人体脂肪细胞中，胰蛋白酶未能改变胰岛素的抗脂解作用，但显着增强了对肾上腺素、去甲肾上腺素和异丙肾上腺素的脂解反应。胰蛋白酶还增加了儿茶酚胺引起的细胞内 cAMP 积累率。在这些条件下，胰蛋白酶处理的人类脂肪细胞对脂肪分解剂二丁酰 cAMP 具有正常反应。人脂肪细胞明显不同于大鼠脂肪细胞，因为人脂肪细胞膜中存在胰蛋白酶敏感成分，该成分抑制儿茶酚胺诱导的脂肪分解过程并且不同于α。受体。
Trypsin-treated human and rat fat cells were obtained by digestion of adipose tissue with collagenase plus trypsin and their lipolytic response to insulin, catecholamines and dibutyryl cyclic[c]AMP were compared with the lipolytic response of human and rat fat cells isolated with collagenase only. In both human and rat fat cells, no significant modification occurred in the intracellular lactate dehydrogenase content and in the basal release of glycerol after trypsination. In rat fat cells, trypsin abolished the antilipolytic effect of insulin but maintained a normal lipolytic response to epinephrine, norepinephrine and isoproterenol. In human fat cells, on the contrary, trypsin failed to modify the antilipolytic effect of insulin, but markedly potentiated the lipolytic response to epinephrine, norepinephrine and isoproterenol. Trypsin also increased the rate of intracellular cAMP accumulation in response to catecholamines. Under these conditions, trypsin-treated human fat cells had a normal response to the lipolytic agent dibutyryl cAMP. Human fat cells apparently differ from the rat ones by the existence in human adipocyte membranes of a trypsin-sensitive component which inhibits the catecholamine induced lipolytic process and which is different from the .alpha. receptors.