CD40 signaling activated by agonistic anti-CD40 monoclonal antibody 5C11 has different effects on biological behavior of gastric carcinoma cells
CD40 signaling activated by agonistic anti-CD40 monoclonal antibody 5C11 has different effects on biological behavior of gastric carcinoma cells
复制标题
激动性抗CD40单克隆抗体5C11激活的CD40信号对胃癌细胞生物学行为有不同影响
DOI：
10.1016/j.imlet.2010.02.007
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发表时间：
2010-07-08
影响因子：
4.4
通讯作者：
Zhang Xue-guang
中科院分区：
文献类型：
--
作者：
Li Rui;Chen Wei-Chang;Zhang Xue-guang
胃癌细胞系AGS具有高水平的CD 40表达。CD 40存在于多种细胞的表面，包括B细胞、内皮细胞、树突状细胞和一些癌细胞，并且递送调节多种细胞应答的信号，如增殖、分化、生长抑制、细胞死亡。本研究采用半定量RT-PCR方法检测了胃癌细胞系AGS、BGC-823、HGC-27、SGC-7901中CD 40的表达及其转录水平。还使用不同浓度的抗CD 40单克隆抗体（mAb）5C 11进行癌细胞增殖抑制测定。结果表明，单独的5C 11和组合的5C 11、IFN-α和5-FU的处理均显著抑制癌细胞增殖。5C 11、IFN-alpha和5-FU联合应用对人胃癌AGS细胞的生长抑制具有协同作用。结果表明，人源化抗CD 40单克隆抗体5C 11具有良好的临床应用价值。(C)2010爱思唯尔有限公司版权所有。
A gastric cancer cell line, AGS has high-level expression of CD40. CD40 is present on the surfaces of a large variety of cells, including B cells, endothelial cells, dendritic cells and some carcinoma cells, and delivers signals regulating diverse cellular responses, such as proliferation, differentiation, growth suppression, cell death. In this research, the expression of CD40 and CD40 transcription in gastric cancer cell lines (AGS, BGC-823, HGC-27, SGC-7901) was investigated by semi-quantitative RT-PCR. Cancer cell proliferation inhibitory assay was also performed using different concentrations of anti-CD40 monoclonal antibody (mAb), 5C11. Results indicated that treatment of 5C11 alone and combined 5C11, IFN-alpha and 5-FU both significantly inhibited cancer cell proliferation. The synergistic effects of combined 5C11, IFN-alpha and 5-FU on growth inhibition of human gastric cancer AGS cells could be observed. These results suggest that humanized anti-CD40 monoclonal antibody (mAb), 5C11 can be used as a valuable reagent for clinical application. (C) 2010 Elsevier B.V. All rights reserved.