Correlation of tocopherol, tocotrienol, γ-oryzanol and total polyphenol content in rice bran with different antioxidant capacity assays

Correlation of tocopherol, tocotrienol, γ-oryzanol and total polyphenol content in rice bran with different antioxidant capacity assays
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DOI:
10.1016/j.foodchem.2006.07.012
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发表时间:
2007-01-01
期刊:
影响因子:
8.8
通讯作者:
Gavino, Victor C.
Gavino, Victor C.
中科院分区:
农林科学1区
文献类型:
--
作者:
Aguilar-Garcia, Carlos;Gavino, Grace;Gavino, Victor C.

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探讨了米糠粉和糙米粉中各种抗氧化剂含量与其抗氧化能力的关系。用铁还原抗氧化力、2,2‘-azinobis-3-ethylbenzotiazoline-6-sulphonic酸和氧自由基吸收能力测定了3个不同品种的委内瑞拉大米,即西马龙、泽塔15和FONAIAP-1。结果表明,不同水稻品种中总多酚、γ-谷维素、α-生育酚、γ-生育酚、α-生育三烯醇、γ-生育三烯醇和β-生育三烯醇的含量及其组合不同。与糙米粉相比,米皮含有大部分抗氧化剂,相应地具有更高的抗氧化能力。主成分分析和多元回归分析表明,FRAP对多酚和总生育三烯酚敏感,而ORAC对多酚和总生育酚敏感。ABTS是所有检测方法中灵敏度最低的。因此,必须谨慎地解释抗氧化能力分析的结果,特别是在复杂的体系中,有必要进行进一步的研究,以更准确地确定相关化学反应的性质。(C)2006爱思唯尔有限公司。保留所有权利。
The relationship between antioxidant capacity and levels of various antioxidants in rice bran and brown rice powder was evaluated. Three different varieties of Venezuelan rice, namely, Cimarron, Zeta 15 and FONAIAP-1, were studied using ferric reducing antioxidant power (FRAP), 2,2'-azinobis-3-ethylbenzotiazoline-6-sulphonic acid (ABTS), and oxygen radical absorbance capacity (ORAC) to measure antioxidant capacity. The results showed that rice varieties contained different levels and combinations of total polyphenols, gamma-oryzanol, alpha- and gamma-tocopherols and alpha-, gamma- and delta-tocotrienols. Compared to brown rice powder, rice bran contained most of the antioxidants and had correspondingly higher values of antioxidant capacity. Principal components analysis and multiple regression on the data indicate that FRAP was sensitive to polyphenols and total tocotrienols, while ORAC was sensitive to polyphenols and total tocopherols. ABTS was the least sensitive of all assays tested. Thus, results from antioxidant capacity assays must be interpreted with caution particularly in complex systems and that further study is necessary to define more precisely the nature of the relevant chemical reactions. (c) 2006 Elsevier Ltd. All rights reserved.