A conductive ormosil encapsulated with ferrocene conjugate and multiwall carbon nanotubes for biosensing application
A conductive ormosil encapsulated with ferrocene conjugate and multiwall carbon nanotubes for biosensing application
复制标题
DOI：
10.1016/j.biomaterials.2005.07.018
复制
发表时间：
2006-10-01
期刊：
影响因子：
14
通讯作者：
Ju, HX
中科院分区：
文献类型：
--
作者：
Kandimalla, VB;Tripathi, VS;Ju, HX
以3-氨丙基三乙氧基硅烷和2-（3，4-环氧环己基）乙基三甲氧基硅烷为原料，通过掺杂二茂铁壬基甲酸-牛血清白蛋白（FMC-BSA）共轭物和多壁碳纳米管（MWNTs）制备了高无毒、高导电性的有机硅复合膜。以葡萄糖氧化酶（GOD）为模型酶，该膜可用于设计电流型葡萄糖生物传感器。包埋的FMC-BSA偶联物表现出良好的氧化还原电化学性能，固定化GOD具有较高的稳定性。在最佳条件下，该传感器对葡萄糖的检测限为20 μ m（S/N = 3），线性范围为0.05- 20.0mM，比间歇安培法宽，分析时间为25 s。膜的k（M）（IPP）值为6.6 mM，三种组分的接近增强了膜与电极之间的电子传递。该膜可有效地用于其他氧化还原生物活性化合物的包埋和生物传感/生物电化学应用。(c)2005爱思唯尔有限公司保留所有权利。
Highly non-toxic and conductive ormosil composite film was prepared using (3-aminopropyl)triethoxysilane and 2-(3,4-epoxvcvclohexyl)-ethyltrimethoxysilane by doping with ferrocenernonocarboxylic acid-bovine serum albumin (FMC-BSA) conjugate and multiwall carbon nanotubes (MWNTs). With glucose oxidase (GOD) as a model enzyme this film could be used to design an amperometric biosensor for glucose determination. The entrapped FMC-BSA conjugate performed excellent redox electrochemistry and the immobilized GOD was highly stable. Under optimal conditions this biosensor was able to detect glucose with a detection limit of 20 mu m (S/N = 3) in the linear range of 0.05-20.0 mM in flow system, which was wider than the batch amperometric mode, with an analysis time of 25 s for each sample. The value of k(M)(IPP) as 6.6 mM. The proximity of these three components FMC-BSA, MWNTs and GOD enhanced the electron transfer between the film and electrode. This film could be used efficiently for the entrapment of other redox bioactive compounds and biosensing/bioelectrochemical applications. (c) 2005 Elsevier Ltd. All rights reserved.