THE ONTOGENY OF LUTEINIZING-HORMONE-RELEASING HORMONE (LHRH) PRODUCING NEURONS IN THE CHICK-EMBRYO - POSSIBLE EVIDENCE FOR MIGRATING LHRH NEURONS FROM THE OLFACTORY EPITHELIUM EXPRESSING A HIGHLY POLYSIALYLATED NEURAL CELL-ADHESION MOLECULE
THE ONTOGENY OF LUTEINIZING-HORMONE-RELEASING HORMONE (LHRH) PRODUCING NEURONS IN THE CHICK-EMBRYO - POSSIBLE EVIDENCE FOR MIGRATING LHRH NEURONS FROM THE OLFACTORY EPITHELIUM EXPRESSING A HIGHLY POLYSIALYLATED NEURAL CELL-ADHESION MOLECULE
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DOI：
10.1016/0168-0102(91)90073-8
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发表时间：
1991-11-01
影响因子：
2.9
通讯作者：
ARAI, Y
中科院分区：
文献类型：
--
作者：
MURAKAMI, S;SEKI, T;ARAI, Y
用免疫组织化学方法研究了鸡胚从胚胎3.5天（艾德）到出壳日龄表达促黄体生成激素释放激素（LHRH）神经元的发育。在艾德-3.5，LHRH免疫反应阳性神经元首先检测到嗅窝的内侧上皮，但其在大脑中的出现延迟到艾德-4.5。在ED-6-7，LHRH免疫反应阳性细胞的索穿过鼻中隔向腹内侧前脑延伸与嗅神经。用LHRH和神经细胞粘附分子（NCAM-H）的高度唾液酸化形式进行双重染色，发现嗅觉-前脑系统中的LHRH阳性神经元呈强NCAM-H阳性。在艾德-8时，观察到嗅上皮中LHRH阳性细胞数量显著减少，同时前脑区LHRH阳性细胞数量增加。从艾德-11到孵化日，大多数LHRH阳性神经元趋向于移动到它们通常的成人位置，而LHRH阳性细胞在嗅上皮中几乎消失。未发现LHRH免疫反应神经元对NCAM-H呈强阳性。这些结果表明，LHRH神经元起源于嗅基板，然后随着它们的发育，它们穿过鼻中隔并与嗅神经一起进入前脑。NCAM-H与LHRH神经元的发育密切相关，提示NCAM-H可能在引导LHRH神经元从嗅上皮向前脑迁移中起一定作用。
The development of neurons expressing luteinizing hormone-releasing hormone (LHRH) has been studied immunohistochemically in the chick embryo from the 3.5 embryonic day (ED) to the day of hatching. At ED-3.5, LHRH-immunoreactive neurons were first detected in the medial epithelium of the olfactory pit, but their appearance in the brain was delayed to ED-4.5. On EDs-6-7, cords of the LHRH-immunoreactive cells extended across the nasal septum towards the ventromedial forebrain with the olfactory nerve. By double staining for LHRH and a highly polysialylated form of neural cell adhesion molecule (NCAM-H), the LHRH-positive neurons in the olfactory-forebrain system were found strongly NCAM-H-positive. At ED-8, a marked decrease in the number of LHRH-positive cells in the olfactory epithelium and a concomitant increase in the LHRH-positive cells in the forebrain area were noted. From ED-11 to the day of hatching, the majority of LHRH-positive neurons tended to move into their usual adult position, whereas the LHRH-positive cells had almost disappeared in the olfactory epithelium. No LHRH-immunoreactive neurons were found strongly positive to NCAM-H. These results suggest that LHRH neurons originate from the olfactory placode, then as they develop they migrate across the nasal septum and enter the forebrain with the olfactory nerve. The close association of NCAM-H with the developing LHRH neurons raises the possibility that NCAM-H plays some role in guiding the migrating LHRH neurons from the olfactory epithelium to the forebrain.