A Quantitative Method for Evaluating Phragmoplast Morphology
A Quantitative Method for Evaluating Phragmoplast Morphology
复制标题
评价成膜体形态的定量方法
DOI：
10.1007/978-1-0716-1744-1_13
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Oda Yoshihisa
中科院分区：
文献类型：
--
作者：
Sasaki Takema;Oda Yoshihisa
成膜体是植物特有的微管结构，在细胞分裂平面形成细胞板。在后期后期，成膜体作为纺锤体微管的衍生物出现在子核之间，并在末期向细胞皮层离心扩张以构建细胞板。成膜体由短的反平行微管组成，上面装饰有各种微管相关蛋白。这些微管相关蛋白的突变体表现出成膜体形态缺陷。成膜体形态的量化对于评估这些突变体的表型是必不可少的。在这里，我们描述了一种量化成膜体宽度的方法。
Phragmoplasts are plant-specific microtubule structures that form cell plates at the cell division plane. During late anaphase, phragmoplasts emerge between daughter nuclei as the derivative of spindle microtubules, and centrifugally expand toward the cell cortex to build cell plates during telophase. Phragmoplasts are composed of short antiparallel microtubules decorated with various microtubule-associated proteins. Mutants of these microtubule-associated proteins exhibit defects in phragmoplast morphology. Quantification of phragmoplast morphology is indispensable for assessing the phenotypes of these mutants. Here, we describe a method to quantify the width of phragmoplasts.