Gellan gum-based hydrogels for intervertebral disc tissue-engineering applications
Gellan gum-based hydrogels for intervertebral disc tissue-engineering applications
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DOI：
10.1002/term.363
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发表时间：
2011-06-01
影响因子：
3.3
通讯作者：
Reis, R. L.
中科院分区：
文献类型：
--
作者：
Silva-Correia, J.;Oliveira, J. M.;Reis, R. L.
椎间盘（IVD）退变是一个具有挑战性的临床问题，迫切需要可行的髓核（NP）植入材料。最适合纳米粒子再生的生物材料尚未确定，但据信可生物降解的水凝胶基材料是有前途的候选材料。在这项工作中，我们开发了离子交联和光交联的甲基丙烯酸结冷胶 (GG-MA) 水凝胶，用于 IVD 再生的非细胞和细胞组织工程策略。通过傅里叶变换红外光谱、H-1核磁共振和差示扫描量热法研究了所开发的水凝胶的物理化学性质。还在生理 pH 值的磷酸盐缓冲盐溶液中分析了水凝胶的溶胀能力和降解率 30 天。此外，分别在扫描电子显微镜和动态压缩下评估了水凝胶的形态和机械性能。进行了一项体外研究，通过用水凝胶浸出物培养大鼠肺成纤维细胞（L929 细胞）长达 7 天来筛选基于结冷胶的水凝胶可能的细胞毒性。结果表明结冷胶成功甲基丙烯酸化。我们观察到，与结冷胶相比，所生产的 GG-MA 水凝胶具有改善的机械性能、较低的吸水能力和降解率。这项工作还表明，GG-MA 水凝胶在体外无细胞毒性，因此有望成为用于 IVD 组织工程策略的生物材料。版权。 (C) 2010 约翰威利父子公司。
Intervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it is believed that biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic-and photo-crosslinked methacrylated gellan gum (GG-MA) hydrogels to be used in acellular and cellular tissue-engineering strategies for the regeneration of IVDs. The physicochemical properties of the developed hydrogels were investigated by Fourier-transform infrared spectroscopy, H-1 nuclear magnetic resonance and differential scanning calorimetry. The swelling ability and degradation rate of hydrogels were also analysed in phosphate-buffered saline solution at physiological pH for a period of 30 days. Additionally, the morphology and mechanical properties of the hydrogels were assessed under a scanning electron microscope and dynamic compression, respectively. An in vitro study was carried out to screen possible cytotoxicity of the gellan gum-based hydrogels by culturing rat lung fibroblasts (L929 cells) with hydrogel leachables up to 7 days. The results demonstrated that gellan gum was successfully methacrylated. We observed that the produced GG-MA hydrogels possess improved mechanical properties and lower water uptake ability and degradation rate as compared to gellan gum. This work also revealed that GG-MA hydrogels are non-cytotoxic in vitro, thus being promising biomaterials to be used in IVD tissue-engineering strategies. Copyright. (C) 2010 John Wiley & Sons, Ltd.