H3K4me2 functions as a repressive epigenetic mark in plants
H3K4me2 functions as a repressive epigenetic mark in plants
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H3K4me2 在植物中充当抑制性表观遗传标记
DOI：
10.1186/s13072-019-0285-6
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发表时间：
2019-07-02
影响因子：
3.9
通讯作者：
Dong, Aiwu
中科院分区：
文献类型：
--
作者：
Liu, Yuhao;Liu, Kunpeng;Dong, Aiwu
BackgroundIn动物，H3 K4 me 2和H3 K4 me 3是丰富的转录起始位点（TSS）和功能作为表观遗传标记，调节基因转录，但它们在植物中的功能还没有得到充分的characteristic.ResultsWe染色质免疫沉淀测序分析水稻全基因组变化H3 K4 me 1/H3 K4 me 2/H3 K4 me 3后的损失H3 K4特异性甲基转移酶，SDG 701。SDG 701的敲低导致整个水稻基因组中H3 K4 me 2/H3 K4 me 3水平的整体降低。RNA测序分析显示，许多与不同发育过程相关的基因在SDG 701敲除突变体中被错误调控。在水稻中，H3 K4 me 3和H3 K36 me 3与基因转录正相关;然而，令人惊讶的是，H3 K4 me 2水平与基因转录水平负相关。此外，TSS区域的H3 K4 me 3水平在SDG 701敲除突变体中表现出下调表达的基因中显著降低。与此相反，在突变体中表达上调的基因与H3 K4 me 2的水平在基因体region.ConclusionA比较的H3 K4 me 2在真核生物的全基因组分布表明，H3 K4 me 2的水平是不相关的基因转录水平在酵母中，但正相关和负相关的基因表达在动物和植物中，分别。我们的研究结果揭示了H3 K4 me 2作为一种新的植物抑制标记。
BackgroundIn animals, H3K4me2 and H3K4me3 are enriched at the transcription start site (TSS) and function as epigenetic marks that regulate gene transcription, but their functions in plants have not been fully characterized.ResultsWe used chromatin immunoprecipitation sequencing to analyze the rice genome-wide changes to H3K4me1/H3K4me2/H3K4me3 following the loss of an H3K4-specific methyltransferase, SDG701. The knockdown ofSDG701resulted in a global decrease in H3K4me2/H3K4me3 levels throughout the rice genome. An RNA-sequencing analysis revealed that many genes related to diverse developmental processes were misregulated in theSDG701knockdown mutant. In rice, H3K4me3 and H3K36me3 are positively correlated with gene transcription; however, surprisingly, the H3K4me2 level was negatively associated with gene transcription levels. Furthermore, the H3K4me3 level at the TSS region decreased significantly in the genes that exhibited down-regulated expression in theSDG701knockdown mutant. In contrast, the genes with up-regulated expression in the mutant were associated with a considerable decrease in H3K4me2 levels over the gene body region.ConclusionA comparison of the genome-wide distributions of H3K4me2 in eukaryotes indicated that the H3K4me2 level is not correlated with the gene transcription level in yeast, but is positively and negatively correlated with gene expression in animals and plants, respectively. Our results uncovered H3K4me2 as a novel repressive mark in plants.