Survival of Staphylococcus epidermidis in Fibroblasts and Osteoblasts
Survival of Staphylococcus epidermidis in Fibroblasts and Osteoblasts
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DOI：
10.1128/iai.00237-18
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发表时间：
2018-10-01
影响因子：
3.1
通讯作者：
Patel, Robin
中科院分区：
文献类型：
--
作者：
Perez, Kimberly;Patel, Robin
表皮葡萄球菌是与留置医疗器械相关的感染的主要原因，包括假体关节感染。虽然生物膜的形成被认为是表皮葡萄球菌引起慢性感染的主要机制，但我们假设表皮葡萄球菌也逃避免疫杀伤，这是其发病机制之一。在这里，我们展示了假体关节相关的表皮葡萄球菌分离株可以在人成纤维细胞内以及人和小鼠成骨细胞内存活。我们还表明，细胞内持续存在的细菌主要居住在酸性吞噬溶菌体中，在感染过程中，选择了小菌落变体。此外，在真核细胞死亡后，这些细菌可以比宿主存活更长的时间，进入细胞外环境，为它们提供机会在种植体相关感染的延迟时间点在种植体表面形成生物膜。综上所述，成纤维细胞和成骨细胞的酸性吞噬小体可作为慢性或迟发性表皮葡萄球菌感染的蓄水池。
Staphylococcus epidermidis is a leading cause of infections associated with indwelling medical devices, including prosthetic joint infection. While biofilm formation is assumed to be the main mechanism underlying the chronic infections S. epidermidis causes, we hypothesized that S. epidermidis also evades immune killing, contributing to its pathogenesis. Here, we show that prosthetic joint-associated S. epidermidis isolates can persist intracellularly within human fibroblasts and inside human and mouse osteoblasts. We also show that the intracellularly persisting bacteria reside primarily within acidic phagolysosomes and that over the course of infection, small-colony variants are selected for. Moreover, upon eukaryotic cell death, these bacteria, which can outlive their host, can escape into the extracellular environment, providing them an opportunity to form biofilms on implant surfaces at delayed time points in implant-associated infection. In summary, the acidic phagolysosomes of fibroblasts and osteoblasts serve as reservoirs for chronic or delayed S. epidermidis infection.