The roles of H2S and H2O2 in regulating AsA-GSH cycle in the leaves of wheat seedlings under drought stress
The roles of H2S and H2O2 in regulating AsA-GSH cycle in the leaves of wheat seedlings under drought stress
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DOI：
10.1007/s00709-018-1213-5
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发表时间：
2018-07-01
期刊：
影响因子：
2.9
通讯作者：
Ou, Xingqi
中科院分区：
文献类型：
--
作者：
Shan, Changjuan;Zhang, Shengli;Ou, Xingqi
本文研究了硫化氢（H2S）和过氧化氢（H2O2）在干旱胁迫（DS）下调节小麦叶片AsA-GSH循环中的作用以及它们之间可能的关系。结果表明，DS显着增加了H2S和H2O2的产生，以及抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）、单脱氢抗坏血酸还原酶（MDHAR）和脱氢抗坏血酸还原酶（DHAR）的转录水平和活性；丙二醛（MDA）含量；和电解液泄漏（EL）。同时，DS显着降低了株高和生物量。除MDA含量和EL外，上述由干旱胁迫引起的增加均被H2S合成抑制剂氨氧乙酸(AOA)和H2O2合成抑制剂二亚苯基碘鎓(DPI)预处理所抑制。此外，AOA和DPI预处理进一步显着增加了MDA含量和EL，并显着降低了DS下的株高和生物量。 DPI 减少了 DS 诱导的 H2O2 和 H2S 的产生。 AOA 还减少了 DS 诱导的 H2S 和 H2O2 的产生。 NaHS+AOA和H2O2+DPI预处理逆转了AOA和DPI的上述影响。我们的结果表明，H2S和H2O2都参与了DS对小麦叶片AsA-GSH循环的上调，并且可能相互影响。
This paper investigated the roles of hydrogen sulfide (H2S) and hydrogen peroxide (H2O2) and the possible relationship between them in regulating the AsA-GSH cycle in wheat leaves under drought stress (DS). Results showed that DS markedly increased the production of H2S and H2O2, the transcript levels and activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR); malondialdehyde (MDA) content; and electrolyte leakage (EL). Meanwhile, DS markedly reduced plant height and biomass. Above increases induced by drought stress except MDA content and EL were all suppressed by pretreatments with H2S synthesis inhibitor aminooxyaceticacid (AOA) and H2O2 synthesis inhibitor diphenylene iodonium (DPI). Besides, pretreatments with AOA and DPI further significantly increased MDA content and EL and significantly reduced plant height and biomass under DS. DPI reduced the production of H2O2 and H2S induced by DS. AOA also reduced the production of H2S and H2O2 induced by DS. Pretreatments with NaHS + AOA and H2O2 + DPI reversed above effects of AOA and DPI. Our results suggested that H2S and H2O2 all participated in the up-regulation of AsA-GSH cycle in wheat leaves by DS and possibly affected each other.